Donkey anti-Rat IgG (Chain gamma), (Heavy & Light Chain) Antibody (HRP)

Catalog No. ABIN969723

Quick Overview for Donkey anti-Rat IgG (Chain gamma), (Heavy & Light Chain) Antibody (HRP) (ABIN969723)

Target: IgG

Conjugate: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Product Details

Binding Specificity: Chain gamma, Heavy & Light Chain

Reactivity: Rat

Host: Donkey

Specificity: Based on IEP, this antibody reacts with: heavy (gamma) chains on rat IgG, light chains on all rat immunoglobulins

No Cross-Reactivity: Chicken, Goat, Guinea Pig, Hamster, Horse (Equine), Human, Mouse (Murine), Rabbit, Sheep (Ovine)

Purification: Affinity purified using solid phase Rat IgG (H and L)
Small amounts of intact IgG may be present.

Purity: > 90 % based on SDS-PAGE

Immunogen: Purified Rat IgG, whole molecule

Application Details

Application Notes: This conjugate is suitable for all immunoassay applications.
The optimal working dilution should be determined by the investigator. Suggested starting dilution: 1:500-1:5,000 for Immunohistochemistry. 1:200-1:5000 for ELISA/Western blot

Comment:

Country of Origin: Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Rehydrate with 1.1 mL of deionized water. Allow reconstituted product to stand for at least 30 minutes at room temperature prior to dilution. If necessary, Centrifuge to remove any particulates. Prepare fresh working dilution daily.

Concentration: 1.0 mg/mL

Buffer: 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free

Preservative: Kathon CG

Handling Advice: Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: 4 °C

Target Details

Target: IgG

Target Type: Antibody