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Rabbit anti-Chicken IgY (Heavy & Light Chain) Antibody (HRP)

ELISA, IHC, WB HRP
Catalog No. ABIN969859

Quick Overview for Rabbit anti-Chicken IgY (Heavy & Light Chain) Antibody (HRP) (ABIN969859)

Target

IgY

Conjugate

  • 21
  • 13
  • 11
  • 9
  • 6
  • 6
  • 6
  • 6
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 4
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
HRP

Application

  • 115
  • 107
  • 106
  • 99
  • 43
  • 37
  • 10
  • 6
  • 6
  • 4
  • 4
  • 4
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
  • Binding Specificity

    • 145
    • 6
    • 6
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Heavy & Light Chain

    Reactivity

    • 159
    • 37
    • 3
    • 2
    Chicken

    Host

    • 67
    • 58
    • 44
    • 19
    • 6
    • 4
    • 1
    Rabbit

    Specificity

    Based on IEP, this antibody reacts with: heavy chains on chicken IgG (IgY), light chains on all chicken immunoglobulins

    No Cross-Reactivity

    Chicken

    Purification

    Affinity purified using solid phase Chicken IgG/Y

    Purity

    > 95 % based on SDS-PAGE

    Immunogen

    Purified Chicken IgG/Y (H and L)
  • Application Notes

    This conjugate is suitable for all immunoassay applications.
    The optimal working dilution should be determined by the investigator. Suggested starting dilution: 1:500-1:5,000 for Immunohistochemistry. 1:200-1:5000 for ELISA/Western blot

    Comment

    Country of Origin: Rabbit serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Rehydrate with 1.1 mL of deionized water. Allow reconstituted product to stand for at least 30 minutes at room temperature prior to dilution. If necessary, Centrifuge to remove any particulates. Prepare fresh working dilution daily.

    Concentration

    1.0 mg/mL

    Buffer

    10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free

    Preservative

    Kathon CG

    Handling Advice

    Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

    Storage

    4 °C
  • Target

    IgY

    Alternative Name

    IgG + IgY
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