Goat anti-Human Ig (Chain kappa), (Light Chain) Antibody (Alkaline Phosphatase (AP))

Catalog No. ABIN969902

Quick Overview for Goat anti-Human Ig (Chain kappa), (Light Chain) Antibody (Alkaline Phosphatase (AP)) (ABIN969902)

Target: Ig

Conjugate: Alkaline Phosphatase (AP)

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Product Details

Binding Specificity: Chain kappa, Light Chain

Reactivity: Human

Host: Goat

Specificity: Based on IEP, this antibody reacts with: kappa (κ) light chains on all human immunoglobulins

Cross-Reactivity (Details): Based on IEP, no reactivity is observed to: non-immunoglobulin human serum immunoglobulins and heavy chains on all human immunoglobulins and lambda (λ) light chains on all human immunoglobulins

Characteristics: Concentration: 1.50 mg/mL (E 1 % at 280 nm = 13.0)
Conjugate: Alkaline Phosphatase

Purification: Affinity purified using solid phase human Kappa (κ) Chain

Purity: > 95 % based on SDS-PAGE

Immunogen: Purified human kappa (kappa) light chain

Application Details

Application Notes: This Conjugate is suitable for immunomicroscopy and flow cytometry. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:20 - 1:2,000 for most applications

Comment:

ALP: Enzyme derived from calf intestine (U.S. origin), Catalyzes the hydrolysis of phosphate groups from a substrate, which produces a colored reaction product or a release of light, The most common substrate for ALP is pNPP, which produces a soluble product, Unaffected by biological components in the ELISA assay, and produces consistent results, The reaction rate of ALP is linear, so its detection sensitivity can be increased by increasing the length of the reaction time. Detection OD of 405 nm

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.50 mg/mL

Buffer: 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Prepare working dilution prior to use and then discard.

Storage: 4 °C

Storage Comment: Store undiluted liquid at 2-8 °C. For storage at -20 °C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard.

Target Details

Target: Ig

Alternative Name: Immunoglobulin Ig