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Goat anti-Human Ig (Chain kappa), (Light Chain) Antibody (FITC)

ELISA, FACS, IF, IM FITC
Catalog No. ABIN969906

Quick Overview for Goat anti-Human Ig (Chain kappa), (Light Chain) Antibody (FITC) (ABIN969906)

Target

Ig

Conjugate

  • 54
  • 25
  • 23
  • 21
  • 18
  • 14
  • 12
  • 10
  • 5
  • 4
  • 4
  • 4
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
FITC

Application

  • 172
  • 137
  • 101
  • 69
  • 56
  • 26
  • 22
  • 12
  • 12
  • 7
  • 7
  • 5
  • 4
  • 4
  • 2
  • 1
  • 1
  • 1
ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunomicroscopy (IM)
  • Binding Specificity

    • 79
    • 77
    • 63
    • 38
    • 4
    • 2
    • 1
    Chain kappa, Light Chain

    Reactivity

    • 117
    • 63
    • 20
    • 18
    • 9
    • 4
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human

    Host

    • 101
    • 77
    • 39
    • 18
    • 3
    • 1
    • 1
    Goat

    Specificity

    Based on IEP, this antibody reacts with: kappa (κ) light chains on all human immunoglobulins

    Cross-Reactivity (Details)

    Based on IEP, no reactivity is observed to: non-immunoglobulin human serum immunoglobulins and heavy chains on all human immunoglobulins and lambda (λ) light chains on all human immunoglobulins

    Characteristics

    Concentration: 1.50 mg/mL (E 1 % at 280 nm = 13.0)
    Conjugate: Fluorescein-5-isothiocyanate (FITC) Amax = 494 nm, Emax = 518 nm

    Purification

    Affinity purified using solid phase human Kappa (κ) Chain

    Purity

    > 95 % based on SDS-PAGE

    Immunogen

    Purified human kappa (kappa) light chain
  • Application Notes

    This Conjugate is suitable for immunomicroscopy and flow cytometry. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:20 - 1:2,000 for most applications

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1.50 mg/mL

    Buffer

    10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free

    Preservative

    Sodium azide

    Precaution of Use

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Prepare working dilution prior to use and then discard.

    Storage

    4 °C

    Storage Comment

    2-8 °C
  • Target

    Ig

    Alternative Name

    Immunoglobulin Ig
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