Goat anti-Human Ig (Chain kappa), (Light Chain) Antibody (HRP)

Catalog No. ABIN969908

Quick Overview for Goat anti-Human Ig (Chain kappa), (Light Chain) Antibody (HRP) (ABIN969908)

Target: Ig

Conjugate: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Product Details

Binding Specificity: Chain kappa, Light Chain

Reactivity: Human

Host: Goat

Specificity: Based on IEP, this antibody reacts with: kappa (κ) light chains on all human immunoglobulins

Cross-Reactivity (Details): Based on IEP, no reactivity is observed to: non-immunoglobulin human serum immunoglobulins and heavy chains on all human immunoglobulins and lambda (λ) light chains on all human immunoglobulins

Characteristics: Concentration: 1.0 mg/mL (E 1 % at 280 nm = 13.0)
Conjugate: Horseradish Peroxidase

Purification: Affinity purified using solid phase human Kappa (κ) Chain

Purity: > 95 % based on SDS-PAGE

Immunogen: Purified human kappa (kappa) light chain

Application Details

Application Notes: This Conjugate is suitable for immunomicroscopy and flow cytometry. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:20 - 1:2,000 for most applications

Comment:

HRP: Enzyme derived from the plant horseradish, A small molecule that does not cause steric hindrance in antibody-antigen reactions, Highly sensitive, can detect low levels of antibody and can be used in a sensitive ECL assay, Hydrogen peroxide oxidizes chromogenic substrates such as TMB, ABTS, and OPD to produce a soluble product with a distinct color which can be measured by spectrophotometer, The reaction product has a high color stability after the reaction is stopped. Detection OD of 450 nm

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Rehydrate with 0.55 mL of deionized water and let stand 30 minutes at room temperature to dissolve. Centrifuge to remove any particulates. Prepare fresh working dilution daily.

Concentration: 1.0 mg/mL

Buffer: 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free

Preservative: Kathon CG

Storage: 4 °C

Storage Comment: Store freeze-dried powder at 2-8 °C. Store lyophilized material at 2-8 °C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20 °C as a liquid.

Target Details

Target: Ig

Alternative Name: Immunoglobulin Ig