anti-Holocytochrome C Synthase (HCCS) Antibodies

The protein encoded by HCCS is an enzyme that covalently links a heme group to the apoprotein of cytochrome c. Additionally we are shipping Holocytochrome C Synthase Kits (17) and Holocytochrome C Synthase Proteins (8) and many more products for this protein.

list all antibodies Gene Name GeneID UniProt
HCCS 3052 P53701
HCCS 317444  
HCCS 15159 P53702
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Top anti-Holocytochrome C Synthase Antibodies at antibodies-online.com

Showing 10 out of 83 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Supplier Delivery Price Details
Cow Rabbit Un-conjugated WB WB Suggested Anti-HCCS AntibodyTitration: 1.0 µg/mL <br /> Positive Control: HepG2 Whole Cell 100 μL Log in to see 2 to 3 Days
$289.00
Details
Dog Rabbit Un-conjugated WB WB Suggested Anti-HCCS AntibodyTitration: 1.0 µg/mL <br /> Positive Control: U937 Whole Cell 100 μL Log in to see 2 to 3 Days
$289.00
Details
Human Rabbit Un-conjugated IF, IHC, ELISA, WB Western blot analysis of extracts from HeLa/HepG2/COLO cells, using Cytochrome c-type Heme Lyase Antibody. The lane on the right is treated with the synthesized peptide. Immunohistochemistry analysis of paraffin-embedded human tonsil tissue, using Cytochrome c-type Heme Lyase Antibody. The picture on the right is treated with the synthesized peptide. 100 μg Log in to see 2 to 3 Days
$302.50
Details
Human Rabbit Un-conjugated IHC (p), WB Western blot analysis of HCCS Antibody (Center) (ABIN653839) in WiDr cell line lysates (35 µg/lane). HCCS (arrow) was detected using the purified polyclonal antibody. Western blot analysis of HCCS Antibody (Center) (ABIN653839) in mouse heart tissue lysates (35 µg/lane). HCCS (arrow) was detected using the purified polyclonal antibody. 400 μL Log in to see 10 to 11 Days
$324.50
Details
Human Rabbit Un-conjugated IHC (p), WB Immunohistochemistry analysis in formalin fixed and paraffin embedded colon carcinoma reacted with HCCS / CCHL Antibody (Center) followed which was peroxidase conjugated to the secondary antibody and followed by DAB staining. Western blot analysis of HCCS / CCHL Antibody (Center) in mouse heart tissue lysates (35ug/lane). HCCS (arrow) was detected using the purified Pab. 0.4 mL Log in to see 6 to 8 Days
$390.50
Details
Hamster Rabbit Un-conjugated WB 50 μg Log in to see 7 to 9 Days
$551.83
Details
Human Rabbit Un-conjugated ICC, IF, IHC, IHC (p), WB Immunocytochemistry/Immunofluorescence: HCCS Antibody [NBP1-86577] - Staining of human cell line U-251MG shows positivity in mitochondria. Western Blot: HCCS Antibody [NBP1-86577] - Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 18.Lane 2: Human cell line RT-4 0.1 mL Log in to see 10 to 13 Days
$439.69
Details
Human Rabbit Un-conjugated ELISA, IF, IHC, WB 100 μL Log in to see 16 Days
$175.80
Details
Human Rabbit Un-conjugated IF (p), IHC (p) Paraformaldehyde-fixed, paraffin embedded rat brain tissue, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30min, Antibody incubation with HCCS Polyclonal Antibody, Unconjugated  at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining. 100 μL Log in to see 14 to 21 Days
$295.90
Details
Human Rabbit Un-conjugated IC, IF, IP, IHC, WB Immunofluorescent analysis of HCCS staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Immunohistochemical analysis of HCCS staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. 200 μL Log in to see 13 to 14 Days
$487.50
Details

HCCS Antibodies by Reactivity, Application, Clonality and Conjugate

Attributes Applications Host Clonality Conjugate
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Top referenced anti-Holocytochrome C Synthase Antibodies

  1. Human Polyclonal HCCS Primary Antibody for ICC, IF - ABIN4316664 : Thinon, Serwa, Broncel, Brannigan, Brassat, Wright, Heal, Wilkinson, Mann, Tate: Global profiling of co- and post-translationally N-myristoylated proteomes in human cells. in Nature communications 2014 (PubMed)

More Antibodies against Holocytochrome C Synthase Interaction Partners

Human Holocytochrome C Synthase (HCCS) interaction partners

  1. Data suggest that although HCCS mediates heme attachment to N-terminal cysteine in heme-attachment site (CXXXH) of cytochrome C (show CYCS Antibodies) variants, up to 50% of cytochrome C (show CYCS Antibodies) produced is modified in an oxygen-dependent manner, resulting in a mixed population of cytochrome c (show CYCS Antibodies). [HCCS = holocytochrome c synthase]

  2. Bacterial cyt c (show CYCS Antibodies) biogenesis pathways (Systems I and II) appear to recognize simply the CXXCH motif, not requiring alpha helix-1. Results here explain mechanistically how HCCS (System III) requires an extended region adjacent to CXXCH for maturation.

  3. cysteines and histidine of the heme attachment site, Cys (show DNAJC5 Antibodies)-XX-Cys (show DNAJC5 Antibodies)-His play a key role in mitochondrial holocytochrome c synthase

  4. These data indicate that heme contacts mediated by residues within these domains modulate the dynamics of heme binding and contribute to the stability of the HCCS-heme-cytochrome c (show CYCS Antibodies) steady state ternary complex.

  5. Identification of a deletion in two patients, including HCCS, leads to the diagnosis of microphthalmia with linear skin defects syndrome

  6. Spectroscopic analyses of HCCS alone and complexes of HCCS with site-directed variants of cytochrome c (show CYCS Antibodies) revealed the fundamental steps of heme attachment and maturation.

  7. Through the study of genetically engineered mice, the loss of HCCS is demonstrated to cause the male lethality of microphthalmia with linear skin defects (MLS) syndrome.

  8. mutant proteins (R217C and Delta 197-268) were unable to complement a Saccharomyces cerevisiae mutant deficient for the HCCS orthologue Cyc3p, in contrast to wild-type HCCS

  9. Missense mutation p.E159K of HCCS, leading to loss-of-function of encoded holocytochrome c-type synthase, in female with microphthalmia of both eyes and bilateral sclerocornea may suggest HCCS as candidate for severe ocular manifestations.

Mouse (Murine) Holocytochrome C Synthase (HCCS) interaction partners

  1. Analysis of Mid1 (show MID1 Antibodies), Hccs, Arhgap6 (show ARHGAP6 Antibodies), and Msl3l1 (show MSL3 Antibodies) in X-linked polydactyly (Xpl) and Patchy-fur (Paf (show KIAA0101 Antibodies)) mutant mice

Holocytochrome C Synthase (HCCS) Antigen Profile

Protein Summary

The protein encoded by this gene is an enzyme that covalently links a heme group to the apoprotein of cytochrome c. Defects in this gene are a cause of microphthalmia syndromic type 7 (MCOPS7). Three transcript variants encoding the same protein have been found for this gene.

Gene names and symbols associated with HCCS

  • holocytochrome c synthase b (hccsb) antibody
  • holocytochrome c synthase (hccs) antibody
  • cytochrome c heme-lyase (cchl) antibody
  • holocytochrome c synthase (HCCS) antibody
  • holocytochrome c synthase (Hccs) antibody
  • cytochrome c-type heme lyase (LOC100356413) antibody
  • holocytochrome c synthetase (Hccs) antibody
  • CCHL antibody
  • DDBDRAFT_0217711 antibody
  • DDBDRAFT_0266707 antibody
  • DDB_0217711 antibody
  • DDB_0266707 antibody
  • hccsb antibody
  • id:ibd1278 antibody
  • MCOPS7 antibody
  • RGD1563855 antibody
  • wu:fb18h01 antibody
  • wu:fc64e12 antibody
  • wu:fi43e01 antibody

Protein level used designations for HCCS

cytochrome c-type heme lyase , holocytochrome c synthetase b , holocytochrome c synthase (cytochrome c heme-lyase) , holocytochrome c synthase , cytochrome c heme-lyase , holocytochrome c-type synthase , holocytochrome c synthetase , CCHL

GENE ID SPECIES
58016 Danio rerio
448226 Xenopus (Silurana) tropicalis
8620147 Dictyostelium discoideum AX4
3052 Homo sapiens
317444 Rattus norvegicus
506250 Bos taurus
424482 Gallus gallus
480834 Canis lupus familiaris
100356413 Oryctolagus cuniculus
15159 Mus musculus
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