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PURB product is a sequence-specific, single-stranded DNA-binding protein. Additionally we are shipping Purine-Rich Element Binding Protein B Proteins (5) and Purine-Rich Element Binding Protein B Kits (3) and many more products for this protein.
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site-directed mutagenesis revealed several basic amino acid residues in the intermolecular (R267) and intramolecular (K82 (show KRT82 Antibodies) and R159) subdomains that are essential for Purbeta transcriptional repressor function in Acta2 (show ACTA2 Antibodies) promoter-reporter assays. In keeping with their diminished Acta2 (show ACTA2 Antibodies) repressor activity in fibroblasts, purified Purbeta variants containing an R267A mutation exhibited reduced binding affinity for purine-ric (show FXYD5 Antibodies)...
This structural arrangement suggests that the cooperative assembly of the dimeric Purbeta repressor on the sense strand of the ACTA2 (show ACTA2 Antibodies) enhancer is dictated by the association of each subdomain with distinct purine-rich binding sites within the enhancer.
These findings suggest that the highly conserved modular repeats of Purbeta fold to form a core functional domain, which mediates the specific and high affinity binding of the protein to single-stranded DNA.
Overexpression of Purbeta, but not Puralpha (show PURA Antibodies), significantly downregulated GnRH1 (show GNRH1 Antibodies) mRNA levels in transiently transfected GT1 (show MYL4 Antibodies)-7 cells, suggesting that Purbeta acts as a repressor of GnRH1 (show GNRH1 Antibodies) gene transcription.
Purbeta is a single-stranded DNA-binding repressor of vascular smooth muscle alpha-actin (show ACTA2 Antibodies) gene transcription
analysis of of DNA binding and localized strand separation by Pur alpha (show PURA Antibodies) and comparison with Pur beta
Pur alpha (show PURA Antibodies) and Pur beta repress smooth muscle alpha-actin (show ACTA2 Antibodies) gene transcription by means of DNA strand-selective cis (show CISH Antibodies)-element binding and cell type-dependent protein-protein interactions
analysis of the quaternary structure of recombinant mouse Purbeta
These data demonstrate that Pur proteins collaborate with Sp3 (show SP3 Antibodies) to regulate a transcriptional program that enables muscle cells to remodel their phenotype.
structural role for certain core arginine residues in forming a conformationally stable Pur beta protein capable of physical interactions necessary for smooth muscle alpha-actin (show ACTA2 Antibodies) gene repression.
Suggest dynamic interplay between transcriptional activators Pur-alpha (show PURA Antibodies)/Pur-beta and repressors in regulating SMalphaA (show ACTA2 Antibodies) gene output during myofibroblast differentiation.
results show that PUR proteins are capable of binding to alpha-MHC (show MYH6 Antibodies) mRNA and attenuate its translational efficiency; also show robust expression of PUR proteins in failing hearts where alpha-MHC (show MYH6 Antibodies) mRNA levels are suppressed
findings offer insights into mechanism, energetics, and sequence of Purbeta repressor binding to a biologically relevant, contractile phenotype-regulating cis (show CISH Antibodies)-element while revealing the thermodynamic confines of Purbeta-mediated effects on DNA structure
This gene product is a sequence-specific, single-stranded DNA-binding protein. It binds preferentially to the single strand of the purine-rich element termed PUR, which is present at origins of replication and in gene flanking regions in a variety of eukaryotes from yeasts through humans. Thus, it is implicated in the control of both DNA replication and transcription. Deletion of this gene has been associated with myelodysplastic syndrome and acute myelogenous leukemia.
purine-rich element-binding protein B
, transcription factor Pur-beta
, transcriptional activator protein Pur-beta
, PurB beta
, purine-rich single-stranded DNA-binding protein beta
, vascular actin single-stranded DNA-binding factor 2 p44 component
, purine-rich element-binding protein B-B
, transcriptional activator protein Pur-beta-B
, purine-rich element binding protein B