Z-FA-FMK, Negative Control for Caspase Inhibitors

Catalog No. ABIN2691059

Product Details

Reactivity: Chemical

Application: Flow Cytometry (FACS)

Brand: BD Pharmingen™

Characteristics: Members of the caspase family play key roles in inflammation and mammalian apoptosis. Z-FA-FMK is a negative control inhibitor which has no inhibitory effect on apoptosis mediated by caspases and can only inhibit cysteine proteases (those not requiring P1 Asp inhibitors) such as Cathepsin B. Z-FA-FMK has a molecular weight of 386 Daltons. Flow cytometric analysis of apoptosis in Jurkat cells (Human T-cell leukemia, ATCC TIB-152). Jurkat cells were preincubated with the following: no inhibitor (upper left and bottom left panels), 20 μM Z-LEHD-FMK (a caspase-9 inhibitor) (upper center and bottom center panels) or 20 μM Z-FA-FMK negative control inhibitor (upper right and bottom right panels) for 30 minutes, and then either left untreated (bottom row) or treated with 4 μM campthothecin for 3 hours (top row). Following incubation, cells were collected and stained with PE Annexin V (Cat. No. 559763) to identify cells undergoing apoptosis. The results indicate that in campthothecin treated cells, approximately 42 % of the cells were induced to undergo apoptosis and the use of the negative control inhibitor, Z-FA-FMK, showed similar results to the treated cells without inhibitor (right panels), indicating that the negative control inhibitor did not attenuate apoptosis.

Purification: Purified

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

BD Pharmingen™ Z-FA-FMK, Negative Control for Caspase Inhibitors - Purified

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstituted Z-FA-FMK may be stored in small aliquots at -20 °C.

Buffer: Lyophilized in dimethyl sulfoxide (DMSO).

Storage: -20 °C

Storage Comment: Avoid multiple freeze-thaws of product. Store lyophilized Z-FA-FMK at -20°C. Reconstitute Z-FA-FMK in DMSO before use. Reconstituted Z-FA-FMK may be stored in small aliquots at -20°C.

References

Gregoli, Bondurant: "Function of caspases in regulating apoptosis caused by erythropoietin deprivation in erythroid progenitors." in: Journal of cellular physiology, Vol. 178, Issue 2, pp. 133-43, (1999) (PubMed).

Thornberry, Lazebnik: "Caspases: enemies within." in: Science (New York, N.Y.), Vol. 281, Issue 5381, pp. 1312-6, (1998) (PubMed).