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GFP (AA 246) antibody
Alternatives ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
|12 references available|
|Quantity||1 mg (1.0 mg/mL)|
|Price||313.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence derived from the jellyfish Aequorea victoria.|
|Description||Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.|
|Characteristics||Concentration Definition: by UV absorbance at 280 nm|
Anti-GFP is designed to detect GFP and its variants. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. Biotin conjugated polyclonal anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution using this antibody in combination with monoclonal anti-GFP (600-301-215) using either form of the antibody as the capture or detection antibody. However, use the monoclonal form only for the detection of wild type or recombinant GFP as this form does not sufficiently detect 'enhanced' GFP. The detection antibody is typically conjugated to biotin and subsequently reacted with streptavidin-HRP (code # S000-03). Fluorochrome conjugated polyclonal anti-GFP can be used to detect GFP by immunofluorescence microscopy in prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and detects GFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-GFP relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP-containing proteins on western blots. Researchers should determine optimal titers for applications.
ELISA Dilution: 1:40,000
Immunohistochemistry Dilution: 1:200 - 1:1,000
Western Blot Dilution: 1:400 - 1:2,000
|Buffer||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Preservative||0.01% (w/v) Sodium azide|
|Storage||Expiration date is one (1) year from date of opening. None. Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.|
|Restrictions||For Research Use only|
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Maher-Laporte, Berthiaume, Moreau et al.: "Molecular composition of staufen2-containing ribonucleoproteins in embryonic rat brain." in: PLoS ONE, Vol. 5, Issue 6, pp. e11350, 2010 (PubMed).
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Justice, Yuan, Sawchenko et al.: "Type 1 corticotropin-releasing factor receptor expression reported in BAC transgenic mice: implications for reconciling ligand-receptor mismatch in the central corticotropin-releasing factor system." in: The Journal of comparative neurology, Vol. 511, Issue 4, pp. 479-96, 2008 (PubMed).
Puthussery, Gayet-Primo, Taylor: "Localization of the calcium-binding protein secretagogin in cone bipolar cells of the mammalian retina." in: The Journal of comparative neurology, Vol. 518, Issue 4, pp. 513-25, 2009 (PubMed).
Hilgen, von Maltzahn, Willecke et al.: "Subcellular distribution of connexin45 in OFF bipolar cells of the mouse retina." in: The Journal of comparative neurology, Vol. 519, Issue 3, pp. 433-50, 2010 (PubMed).
Brett, Renault, Rafalski et al.: "The microRNA cluster miR-106b~25 regulates adult neural stem/progenitor cell proliferation and neuronal differentiation." in: Aging, Vol. 3, Issue 2, pp. 108-24, 2011 (PubMed). Method employed by authors: Immunohistochemistry (IHC) (1:500, Sample species: Mouse (Murine)).