Rabbit anti-Cow IgG (Fc Region) Antibody (TRITC) - Preadsorbed
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- Target See all IgG products
- IgG
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Binding Specificity
- Fc Region
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Reactivity
- Cow
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- TRITC
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Application
- Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
- Specificity
- IgG F(c)
- Characteristics
- Concentration Definition: by UV absorbance at 280 nm
- Purification
- Preadsorption: immunoaffinity chromatography using Bovine IgG coupled to agarose beads
- Immunogen
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Immunogen: Bovine IgG F(c) fragment
- Isotype
- IgG
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- Application Notes
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Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
FLISA Dilution: 1:10,000 - 1:50,000
Flow Cytometry Dilution: 1:500 - 1:2,500
IF Microscopy Dilution: 1:1,000 - 1:5,000
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 2.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- Handling Advice
- Product is photosensitive and should be protected from light.
- Storage
- RT,4 °C,-20 °C
- Expiry Date
- 12 months
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- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
- Background
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Synonyms: rabbit anti-Bovine IgG F(c) Rhodamine Conjugated Antibody, rabbit anti-Bovine IgG Fc Rhodamine Conjugated Antibody
Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. This product possesses the F(c) region, recognized by high-affinity Fc receptor proteins.
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