Chicken anti-Human IgG (Heavy & Light Chain) Antibody (HRP)

Details for Product No. ABIN101472, Supplier: Log in to see
Antigen
Epitope
Heavy & Light Chain
4683
2573
1882
1039
1015
825
305
273
35
34
31
8
7
7
3
3
2
2
1
1
1
1
1
1
Reactivity
Human
2780
2759
2347
1724
1436
778
607
426
364
338
330
324
307
290
169
148
126
114
41
29
27
25
12
11
9
8
6
5
5
4
4
4
4
4
4
4
4
3
3
3
2
2
2
1
1
1
1
1
Host
Chicken
6750
4661
1834
812
683
394
88
52
51
9
8
5
5
4
2
1
1
Clonality
Polyclonal
Conjugate
HRP
2182
1862
1718
1362
765
675
521
297
295
284
274
268
217
186
178
117
102
79
69
58
54
40
35
26
23
22
19
19
19
19
19
16
15
15
15
15
15
15
14
13
12
11
11
10
10
9
9
9
9
9
9
9
8
8
8
7
6
6
6
6
6
6
6
5
5
5
5
5
4
4
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
Options
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Immunogen Human IgG whole molecule
Isotype IgG
Specificity IgG (H&L), Anti-Human IgG (H&L) Antibody Peroxidase Conjugated antibody detects human IgG.
Characteristics Concentration Definition: by UV absorbance at 280 nm
Background Synonyms: anti-human immunoglobulin G
Research Area Immunology, Secondary Antibodies
Application Notes Human IgG (H&L) Antibody is peroxidase conjugated and is suitable for immunoassays where specificity to the immunoglobulin heavy and or light chain regions is desired. Antibody has been tested by ELISA, western blot immunoblot, and immunohistochemisty. Optimal concentrations in immunoassays should be determined by the researcher.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Restore with deionized water (or equivalent)
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Gentamicin sulfate
Handling Advice Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Supplier Images
Western Blotting (WB) image for Chicken anti-Human IgG (Heavy & Light Chain) Antibody (HRP) (ABIN101472) Western Blot of Chicken anti-Human IgG (H&L) Antibody Peroxidase Conjugated. Lane 1: ...
Background publications Farr, Nakane: "Immunohistochemistry with enzyme labeled antibodies: a brief review." in: Journal of immunological methods, Vol. 47, Issue 2, pp. 129-44, 1982 (PubMed).