Chicken anti-Human IgG (Heavy & Light Chain) Antibody (HRP)

Details for Product No. ABIN101472, Supplier: Log in to see
Antigen
Epitope
Heavy & Light Chain
4680
2537
1879
1066
1006
860
298
294
35
34
31
8
7
7
3
3
2
2
1
1
1
1
1
Reactivity
Human
2849
2735
2296
1675
1433
788
613
442
380
379
358
339
291
290
208
138
135
122
41
29
27
25
12
11
9
8
6
5
5
5
4
4
4
4
4
4
4
4
3
3
3
2
2
1
1
1
1
1
Host
Chicken
6705
4831
1795
891
608
388
91
55
52
21
8
5
5
5
2
1
1
Clonality
Polyclonal
Conjugate
HRP
2235
1872
1738
1356
767
682
519
297
264
223
222
200
156
138
128
110
79
75
69
55
51
46
43
40
38
36
34
34
32
29
27
27
27
27
27
27
27
27
22
22
19
19
19
19
15
15
15
15
15
15
14
12
12
12
10
10
9
9
9
9
9
9
9
8
8
8
8
8
7
6
6
6
6
5
5
5
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Immunogen Human IgG whole molecule
Isotype IgG
Specificity IgG (H&L), Anti-Human IgG (H&L) Antibody Peroxidase Conjugated antibody detects human IgG.
Characteristics Concentration Definition: by UV absorbance at 280 nm
Background Synonyms: anti-human immunoglobulin G
Research Area Immunology, Secondary Antibodies
Application Notes Human IgG (H&L) Antibody is peroxidase conjugated and is suitable for immunoassays where specificity to the immunoglobulin heavy and or light chain regions is desired. Antibody has been tested by ELISA, western blot immunoblot, and immunohistochemisty. Optimal concentrations in immunoassays should be determined by the researcher.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Restore with deionized water (or equivalent)
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Gentamicin sulfate
Handling Advice Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Supplier Images
Western Blotting (WB) image for Chicken anti-Human IgG (Heavy & Light Chain) antibody (HRP) (ABIN101472) Western Blot of Chicken anti-Human IgG (H&L) Antibody Peroxidase Conjugated. Lane 1: ...
Background publications Farr, Nakane: "Immunohistochemistry with enzyme labeled antibodies: a brief review." in: Journal of immunological methods, Vol. 47, Issue 2, pp. 129-44, 1982 (PubMed).