IL1A antibody (Cleavage Site)

Catalog No. ABIN102983

Product Details anti-IL1A Antibody

Target: IL1A (Interleukin 1 alpha (IL1A))

Binding Specificity: Cleavage Site

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL1A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Characteristics:

Synonyms: Interleukin 1 alpha precursor antibody, rabbit anti-Interleukin1 alpha antibody, rabbit anti-IL-1 Alpha Antibody, Pre-interleukin 1 alpha antibody

Background: Interleukin 1 alpha is produced by activated macrophages. Anti-IL-1 alpha antibody is ideal for investigators involved in Immunology research.

Gene Name: IL1A

Purification: This is an IgG preparation of whole rabbit serum purified by DEAE fractionation.  This antibody is primarily directed against the 17,000 MW human IL-1a and is useful in determining its presence in various assays.  In general, this antibody also detects primate IL-1a in the same formats using similar dilutions.  The antiserum does not recognize human IL-1b or Mouse or Rabbit IL-1a.  In ELISA formats and other immunoreactive assays, this antibody will recognize both the mature 17,000 MW IL-1a as well as the 31,000 MW IL-1a precursor in either non-denatured (native) or denatured samples.  Unlike the IL-1b precursor, the native precursor of IL-1a is recognized by the antibody produced to the 17,000 MW form.   The 31,000 precursor of IL-1a is biologically active and is found primarily intracellularly.  The precursor of IL-1a, unlike that of IL-1b, is biologically active when applied to cells and is thought to have a role as a functional molecule intracellularly and can be found constitutively expressed in various cell.  This antibody is also useful for neutralization of human and primate IL-1a activity in bioassays.  It does not neutralize the biological activity IL-1b.  It does not neutralize the biological activity of mouse, rat or rabbit IL-1a.  It will neutralize primate IL-a.  For neutralization, it is recommended to incubate the sample with a 1:100 dilution of the antibody for at least 4 hours before being tested.  A control of similarly diluted normal rabbit IgG is recommended.  This antibody can be used for FACS analysis.  Caution should be exhibited as the F( c) domain of the rabbit IgG molecule may interact with cells non-specifically.

Immunogen:

Immunogen: The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with recombinant human IL-1a produced in E.coli.  The MW of the recombinant IL-1a was 17,000.  This is the cleavage site generated by the IL-1b converting enzyme (ICE, capase-1).

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:100 to 1:200

Application Note: This IgG fraction antibody of anti-Human IL-1a has been tested for use in neutralizations, ELISA,  immunohistochemistry, flow cytometry and immunoblotting.  It recognizes the 17,000 MW mature IL-1a.  Reactivity in other immunoassays is unknown.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:1,000 - 1:5,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride,  pH 7.2

Stabilizer: None

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 12 months

Target Details for IL1A

Target: IL1A (Interleukin 1 alpha (IL1A))

Alternative Name: IL-1 Alpha (IL1A Products)

Synonyms: IL-1A antibody, IL1 antibody, IL1-ALPHA antibody, IL1F1 antibody, IL-6 antibody, Il-1a antibody, IL-1 alpha antibody, IL-1alpha antibody, L1A antibody, interleukin 1 alpha antibody, interleukin 6 antibody, IL1A antibody, IL6 antibody, Il1a antibody

Gene ID: 3552

UniProt: P01583

Pathways: NF-kappaB Signaling, Autophagy, Cancer Immune Checkpoints