Leptin (LEP) antibody
| Antigen |
|
| Synonyms |
OB, OBS, FLJ94114, ob, obese |
| Clonality |
Polyclonal |
|
Host
|
|
|
Reactivity
|
|
|
Application
|
 Alternatives Immunohistochemistry (IHC), ELISA, Western Blotting (WB), Immunoprecipitation (IP)
Western Blotting (WB) (76), Immunofluorescence (IF) (57), Flow Cytometry (FACS) (55), Enzyme Immunoassay (EIA) (42), ELISA (34), Immunoprecipitation (IP) (14), Immunohistochemistry (Frozen Sections) (IHC (fro)) (13), Radioimmunoassay (RIA) (12), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (11), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Blocking Antibody (Inhibition) (4), ELISA (Detection) (3), Immunoelectron Microscopy (IEM) (3), ELISA (Capture) (2), Immunocytochemistry (ICC) (2), Transfected cell culture (TCC) (2), Immunohistochemistry (IHC) (1), Neutralization (Neut) (1)
|
| Catalog no. |
ABIN103522 |
| Quantity |
1mg (1.0 mg/ml (by UV absorbance at 280 ...) (Variants) |
| Price |
309.00 $ Plus shipping costs $35.00
|
| Shipping to |
|
| Availability |
Ships within 5 Business Days |
|
Alternative name
|
Leptin
|
|
Immunogen
|
The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with recombinant mouse leptin 16,000 MW produced in E. coli.
|
|
Format
|
Liquid (sterile filtered)
|
|
Specificity
|
This is an IgG preparation of whole rabbit serum purified by Protein G chromatography. The antibody will recognize recombinant and native 16 kDa leptin from mouse. Reactivity with leptin from other sources is unknown. Endotoxin Content: <10 pg/ ul by LAL method.
|
|
Application Notes
|
Recommended Dilutions: This product has been assayed by immunoblot against tissue homogenates using HRP Goat-anti-Rabbit IgG [H&L] (code ABIN101990) and TMB as a substrate. A working dilution range of 1:200 to 1:400 is suggested for this application. This product has been assayed by ELISA against recombinant mouse leptin using HRP Conjugated Anti-Rabbit IgG [H&L] (Goat) (code ABIN101990) and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:2,400 is suggested for this product. Optimal titers for other applications should be determined by the researcher. Note: This IgG fraction of anti-Mouse Leptin has been tested for use in ELISA and immunoblotting. Reactivity in other immunoassays is unknown. Obesity associated leptin is the product of the OB gene and has been identified with Type II diabetes.
|
|
Concentration
|
1.0 mg/ml (by UV absorbance at 280 nm)
|
|
Buffer
|
Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.
|
|
Storage
|
Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is six (6) months from date of opening.
|
|
Research Area
|
Cardiovascular, Atherosclerosis, Hormones
|
|
Restrictions
|
For Research Use only
|
Alternatives for antigen "Leptin (LEP)", type "Antibodies"
|
Hosts
|
Rabbit (118), Mouse (25), Goat (6), Sheep (2)
|
|
Reactivities
|
Human (88), Mouse (Murine) (87), Rat (Rattus) (77), Pig (Porcine) (35), Cow (Bovine) (19), Dog (Canine) (18), Sheep (Ovine) (17), Horse (Equine) (15), Rabbit (2), Chicken (1)
|
|
Applications
|
Western Blotting (WB) (76), Immunofluorescence (IF) (57), Flow Cytometry (FACS) (55), Enzyme Immunoassay (EIA) (42), ELISA (34), Immunoprecipitation (IP) (14), Immunohistochemistry (Frozen Sections) (IHC (fro)) (13), Radioimmunoassay (RIA) (12), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (11), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Blocking Antibody (Inhibition) (4), ELISA (Detection) (3), Immunoelectron Microscopy (IEM) (3), ELISA (Capture) (2), Immunocytochemistry (ICC) (2), Transfected cell culture (TCC) (2), Immunohistochemistry (IHC) (1), Neutralization (Neut) (1)
|
|
Conjugates
|
Biotin (15), Alexa Flour 488 (3), Alexa Fluor 350 (3), Alexa Fluor 488 (3), Alexa Fluor 555 (3), Alexa Fluor 647 (3), Cy3 (3), Cy5 (3), Cy5.5 (3), Cy7 (3), FITC (3), Gold (3), HRP (3), PE (3), PE,Cy3 (3), PE,Cy5 (3), PE,Cy5.5 (3), PE,Cy7 (3), RBITC (3)
|
|
Epitopes
|
C-Term (3), 92-145 cyclized (2), AA 1-24 (2), amino terminal residues 1-24 (2), mouse (1), rat (1), residues 131-145 (1)
|
