CD13 antibody (Alanyl (Membrane) Aminopeptidase) (PerCP)

Details for Product anti-ANPEP Antibody No. ABIN1043023, Supplier: Log in to see
Antigen
  • Apn
  • pepN
  • SC8E4A.13
  • APN
  • CD13
  • GP150
  • LAP1
  • P150
  • PEPN
  • AP-M
  • AP-N
  • Cd13
  • Apm
  • KZP
  • Lap1
  • rAPN
  • APDE
  • p150
  • aminopeptidase N
  • aminopeptidase n
  • alanyl (membrane) aminopeptidase
  • Apn2
  • Apn1
  • SCO2643
  • pepN
  • Shewana3_0897
  • Alvin_1024
  • Ndas_3413
  • Arch_0473
  • Arch_0872
  • Saut_1544
  • Fbal_1877
  • ML5_3424
  • Despr_1988
  • Mesop_5365
  • ANPEP
  • Anpep
Alternatives
anti-Non-Human Primate CD13 antibody for Flow Cytometry
Reactivity
Human, Non-Human Primate
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1
Host
Mouse
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4
Clonality (Clone)
Monoclonal ()
Conjugate
This CD13 antibody is conjugated to PerCP
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62
34
16
11
9
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9
7
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5
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2
2
2
2
1
Application
Flow Cytometry (FACS), Functional Studies (Func), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP)
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84
78
75
37
33
8
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2
2
2
1
1
Options
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Available images

Immunogen Human AML cells
Clone WM15
Isotype IgG1
Specificity The antibody WM15 recognises the human CD13 cell surface glycoprotein, a 150 kDa molecule expressed on granulocytes, endothelial cells, epithelial cells and myeloid progenitors.
HLDA III, WS Code M 213
HLDA IV, WS Code M 44
HLDA IV, WS Code M 209
HLDA V, WS Code M MA191
Characteristics The purified antibody is conjugated with Peridinin-chlorophyll-protein complex (PerCP) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Alternative Name ANPEP (ANPEP Antibody Abstract)
Background CD13 (aminopeptidase N, APN) is a 150 kDa type II transmembrane zinc-binding ectopeptidase expressed on various cell types. This metalloprotease preferentially catalyzes removal of neutral amino acids from small peptides, thus activating or inactivating bioactive peptides. CD13 has also role in extracellular matrix degradation, antigen processing and signal transduction, is important in inflammatory responses, regulates intercellular contact, cell motility and vascularization. CD13 is involved in protection of leukemic cells against apoptosis and its expression associated with poor prognosis of carcinomas.
Gene ID 290
Research Area Surface Receptors of Immune Cells, Hematopoietic Progenitors, Signaling, CD Antigens, Extracellular Matrix, Stem Cells
Pathways Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 10 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (1 ml) is sufficient for 100 tests.
Restrictions For Research Use only
Reconstitution No reconstitution is necessary.
Buffer The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2 % (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Supplier Images
Flow Cytometry (FACS) image for anti-CD13 antibody (Alanyl (Membrane) Aminopeptidase)  (PerCP) (ABIN1043023) Surface staining of human peripheral blood leukocytes with anti-CD13 mouse monoclonal...
Product cited in: McCormack, Muji?, Osdal, Bruserud, Gjertsen: "Multiplexed mAbs: a new strategy in preclinical time-domain imaging of acute myeloid leukemia." in: Blood, Vol. 121, Issue 7, pp. e34-42, 2013 (PubMed).

Favaloro, Browning, Facey: "CD13 (GP150; aminopeptidase-N): predominant functional activity in blood is localized to plasma and is not cell-surface associated." in: Experimental hematology, Vol. 21, Issue 13, pp. 1695-701, 1993 (PubMed).

Bradstock, Favaloro, Kabral, Kerr, Hughes, Berndt, Musgrove: "Human myeloid differentiation antigens identified by monoclonal antibodies: expression on leukemic cells." in: Pathology, Vol. 17, Issue 3, pp. 392-9, 1986 (PubMed).

Bradstock, Favaloro, Kabral, Kerr, Hughes, Musgrove: "Myeloid progenitor surface antigen identified by monoclonal antibody." in: British journal of haematology, Vol. 61, Issue 1, pp. 11-20, 1985 (PubMed).

Background publications Terauchi, Kajiyama, Shibata, Ino, Nawa, Mizutani, Kikkawa: "Inhibition of APN/CD13 leads to suppressed progressive potential in ovarian carcinoma cells." in: BMC cancer, Vol. 7, pp. 140, 2007 (PubMed).

Petrovic, Schacke, Gahagan, OConor, Winnicka, Conway, Mina-Osorio, Shapiro: "CD13/APN regulates endothelial invasion and filopodia formation." in: Blood, Vol. 110, Issue 1, pp. 142-50, 2007 (PubMed).

Tokuhara, Hattori, Ishida, Hirai, Higashiyama, Kodama, Miyake: "Clinical significance of aminopeptidase N in non-small cell lung cancer." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 12, Issue 13, pp. 3971-8, 2006 (PubMed).