Tumor Necrosis Factor (TNF) antibody

Details for Product No. ABIN1043796
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Antigen
Synonyms DIF, TNFA, TNFSF2, TNF-alpha, RATTNF, Tnfa, TNF-a, TNFalpha, Tnfsf1a, TNFa, cTNF, TNF-ALPHA, zgc:55302, wu:fi06b02, TNFAIP1, TNF, tnf, Tnf-alpha, tnfa-like, dif, tnfa, xtnf, tnfsf2, tnf-alpha
Reactivity
Human
(468), (193), (97), (67), (57), (30), (24), (22), (11), (9), (9), (7), (7), (4), (3), (2), (2), (1), (1), (1)
Host
Rabbit
(318), (271), (96), (25), (12), (8), (7), (3), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(85), (43), (23), (8), (7), (6), (6), (5), (4), (4), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Neutralization (Neut)
(434), (386), (188), (114), (102), (84), (64), (64), (53), (53), (39), (31), (30), (19), (15), (8), (5), (5), (5), (4), (3), (3), (3), (3), (2), (1), (1), (1)
Pubmed 1 reference available
Catalog no. ABIN1043796
Quantity 25 µL
Price
126.50 $   Plus shipping costs $45.00 and if applicable $26.40 dry ice
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Immunogen The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with recombinant human TNFalpha produced in E.coli.
Immunogen Type: RecombinantProtein
Isotype IgG
Specificity This antibody is primarily directed against mature 17,000 MW human TNFalpha and is useful in determining its presence in various assays. In general, this antibody also detects primate TNFalpha in the same formats using similar dilutions. The antibody does not recognize human TNGß (lymphotoxin). This IgG fraction antibody will recognize the cell-bound precursor of TNFalpha as a 26,000 protein in immunoblots, particularly in denatured samples. This antibody is also useful for neutralization of human and primate TNFalpha activity in bioassays. It does not neutralize the biological activity of lymphotoxin. For neutralization, it is recommended to incubate the sample with a 1:200 dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended.
Characteristics Anti TNF alpha Antibody recognizes TNF alpha (TNF, cachexin, cachectin, tumor necrosis factor-alpha or TNF-alpha) a cytokine involved in systemic inflammation. TNF alpha is a member of a group of cytokines that stimulate the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by other cell types as well. The primary role of TNF alpha is in the regulation of immune cells. TNFis an endogenous pyrogen that is able to induce fever, apoptotic cell death, sepsis (through IL-1 & IL-6 production), cachexia, inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNF production has been implicated in a variety of human diseases, including Alzheimer's disease, cancer, major depression, and inflammatory bowel disease (IBD).
Sterility Sterile filtered
Alternative Name TNF alpha
Background Anti TNF alpha Antibody recognizes TNF alpha (TNF, cachexin, cachectin, tumor necrosis factor-alpha or TNF-α) a cytokine involved in systemic inflammation. TNF alpha is a member of a group of cytokines that stimulate the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by other cell types as well. The primary role of TNF alpha is in the regulation of immune cells. TNFis an endogenous pyrogen that is able to induce fever, apoptotic cell death, sepsis (through IL-1 & IL-6 production), cachexia, inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNF production has been implicated in a variety of human diseases, including Alzheimer's disease, cancer, major depression, and inflammatory bowel disease (IBD).
Synonyms: APC1 antibody, Cachectin antibody, DIF antibody, Differentiation inducing factor antibody, Macrophage cytotoxic factor antibody, MCF antibody, Necrosin antibody
Gene ID 7124
NCBI Accession P01375.1
UniProt P01375
Research Area Cardiovascular, Atherosclerosis, Growth Factors, Innate Immunity, Cytokines, Receptors, Immunology, Inflammation, Cancer, Virology
Application Notes This IgG fraction antibody of anti-Human TNFα has been tested for use in neutralizations, ELISA, immunohistochemistry and immunoblotting. It recognizes the 17,000 MW TNFα. Reactivity in other immunoassays is unknown.
Comment

Gene Name: TNFA

Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Without preservative
Storage 4 °C/-20 °C
Storage Comment Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  Expiration date is one (1) year from date of opening.
Expiry Date 12 months
Supplier Images
anti-Tumor Necrosis Factor (TNF) antibody Immunohistochemistry using polyclonal TNFa antibody showing staining of formalin/PFA-fixed paraffin-embedded sections of human artery tissue sections. Sections were fixed in formaldehyde and subjected to heat mediated antigen retrieval in citrate buffer (pH 6.0). Slides were blocked for ten minutes with 1.5% serum. Primary antibody was diluted 1:100 and incubated with samples for 24 hours at 4°C. HRP-conjugated goat anti-rabbit antibody was used as the secondary antibody.
anti-Tumor Necrosis Factor (TNF) antibody (2) anti-Tumor Necrosis Factor (TNF) antibody (Image 2)
anti-Tumor Necrosis Factor (TNF) antibody (3) anti-Tumor Necrosis Factor (TNF) antibody (Image 3)
Product cited in: Kant, Swat, Zhang et al.: "TNF-stimulated MAP kinase activation mediated by a Rho family GTPase signaling pathway." in: Genes & development, Vol. 25, Issue 19, pp. 2069-78, 2011 (PubMed).

Hosts (318), (271), (96), (25), (12), (8), (7), (3), (2)
Reactivities (468), (193), (97), (67), (57), (30), (24), (22), (11), (9), (9), (7), (7), (4), (3), (2), (2), (1), (1), (1)
Applications (434), (386), (188), (114), (102), (84), (64), (64), (53), (53), (39), (31), (30), (19), (15), (8), (5), (5), (5), (4), (3), (3), (3), (3), (2), (1), (1), (1)
Conjugates (85), (43), (23), (8), (7), (6), (6), (5), (4), (4), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Epitopes (10), (9), (7), (4), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
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