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Tumor Necrosis Factor (TNF) antibody
Alternatives ELISA, Flow Cytometry (FACS), Fluorescence Microscopy (FM)
|1 reference available|
|Quantity||25 µL (1.0 mg/mL) (Variants)|
|Price||119.90 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Alternative name||TNF alpha|
The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with recombinant human TNFalpha produced in E.coli.
Immunogen Type: Recombinant Protein
This IgG fraction antibody of anti-Human TNFalpha has been tested for use in neutralizations, ELISA, immunohistochemistry and immunoblotting. It recognizes the 17,000 MW TNFalpha. Reactivity in other immunoassays is unknown.
Synonyms: APC1 antibody, Cachectin antibody, DIF antibody, Differentiation inducing factor antibody, Macrophage cytotoxic factor antibody, MCF antibody, Necrosin antibody.
|Characteristics||Anti TNF alpha Antibody recognizes TNF alpha (TNF, cachexin, cachectin, tumor necrosis factor-alpha or TNF-alpha) a cytokine involved in systemic inflammation. TNF alpha is a member of a group of cytokines that stimulate the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by other cell types as well. The primary role of TNF alpha is in the regulation of immune cells. TNFis an endogenous pyrogen that is able to induce fever, apoptotic cell death, sepsis (through IL-1 & IL-6 production), cachexia, inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNF production has been implicated in a variety of human diseases, including Alzheimer's disease, cancer, major depression, and inflammatory bowel disease (IBD).|
|Specificity||This antibody is primarily directed against mature 17,000 MW human TNFalpha and is useful in determining its presence in various assays. In general, this antibody also detects primate TNFalpha in the same formats using similar dilutions. The antibody does not recognize human TNGß (lymphotoxin). This IgG fraction antibody will recognize the cell-bound precursor of TNFalpha as a 26,000 protein in immunoblots, particularly in denatured samples. This antibody is also useful for neutralization of human and primate TNFalpha activity in bioassays. It does not neutralize the biological activity of lymphotoxin. For neutralization, it is recommended to incubate the sample with a 1:200 dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended.|
Gene Name: TNFA
|Synonyms||DIF, TNFA, TNFSF2, TNF-alpha, B12, B61, EDP1, MGC2317, Edp1, Edp-1, Tnfip1, Tnfa, RATTNF, MGC124630, zgc:55302, wu:fi06b02, TNFAIP1, TNF, tnf, DKFZp468A2328, Tnfsf1a, TNFalpha, MGC151434, TNFa, cTNF, Tnf-alpha, tnfa-like, TNF-ALPHA, dif, tnfa, xtnf, TNF-a, tnfsf2, tnf-alpha, Cachectin|
ELISA Dilution: 1:1,000 - 1:5,000.
Western Blot Dilution: 1:500 - 1:2,000.
|Buffer||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Storage Comment||Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.|
|Expiry Date||Expiration date is one (1) year from date of opening.|
|Research Area||Cardiovascular, Atherosclerosis, Growth Factors, Innate Immunity, Cytokines, Receptors, Immunology, Inflammation, Cancer, Virology|
|Restrictions||For Research Use only|
Kant, Swat, Zhang et al.: "TNF-stimulated MAP kinase activation mediated by a Rho family GTPase signaling pathway." in: Genes & development, Vol. 25, Issue 19, pp. 2069-78, 2011 (PubMed).