Chemokine (C-C Motif) Ligand 20 (CCL20) antibody (HRP)

Details for Product No. ABIN1043828
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Antigen
Synonyms MIP-3ALPHA, CCL20, si:dkey-150o13.1, CKb4, LARC, MIP-3-alpha, MIP-3a, MIP3A, SCYA20, ST38, MIP-3A, MIP-3[a], Scya20, exodus-1
Reactivity
Mouse (Murine), Rat (Rattus)
(80), (33), (18), (3), (3)
Host
Rabbit
(56), (32), (10), (6)
Clonality
Polyclonal
Conjugate
HRP
(21), (6), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), ELISA
(70), (55), (29), (14), (10), (10), (9), (8), (8), (8), (3), (3), (3), (2), (2), (2)
Pubmed 1 reference available
Quantity 100 μg
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Catalog No. ABIN1043828
361.90 $
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Immunogen MIP-3 alpha Antibody Peroxidase Conjugated was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant mouse MIP-3alpha protein.
Immunogen Type: Recombinant Protein
Isotype IgG
Specificity Mouse MIP-3 alpha Antibody Peroxidase Conjugated was heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant mouse MIP-3alpha in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
Characteristics MIP-3alpha (also known as C-C motif chemokine 20, small-inducible cytokine A20, macrophage inflammatory protein 3 alpha, MIP-3-alpha, liver and activation-regulated chemokine, CC chemokine LARC and beta chemokine exodus-1) is a chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. MIP-3alpha inhibits proliferation of myeloid progenitors in colony formation assays and may be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. MIP-3alpha also possesses antibacterial activity against E.coli and S.aureus. MIP-3alpha is a secreted protein that is expressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. Low levels of expression are also seen in thymus, prostate, testis, small intestine and colon. C-terminal processed forms which lack 1, 3 or 6 amino acids are produced by proteolytic cleavage after secretion from peripheral blood monocytes.
Purification purified
Alternative Name MIP-3 alpha
Background MIP-3α (also known as C-C motif chemokine 20, small-inducible cytokine A20, macrophage inflammatory protein 3 alpha, MIP-3-alpha, liver and activation-regulated chemokine, CC chemokine LARC and beta chemokine exodus-1) is a chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. MIP-3α inhibits proliferation of myeloid progenitors in colony formation assays and may be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. MIP-3α also possesses antibacterial activity against E.coli and S.aureus. MIP-3α is a secreted protein that is expressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. Low levels of expression are also seen in thymus, prostate, testis, small intestine and colon. C-terminal processed forms which lack 1, 3 or 6 amino acids are produced by proteolytic cleavage after secretion from peripheral blood monocytes.
Synonyms: MIP-3α , CCL20, C-C motif chemokine 20, small-inducible cytokine A20, macrophage inflammatory protein 3 alpha, MIP-3-alpha, liver and activation-regulated chemokine, CC chemokine LARC and beta chemokine exodus-1
Gene ID 20297
NCBI Accession NP_058656
UniProt Q642U4
Application Notes This purified antibody has been tested for use in ELISA and western blotting. By western blot a band approximately 10.7 kDa in size corresponding to mouse MIP-3α protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Comment

Gene Name: Ccl20

Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative Gentamicin sulfate
Handling Advice Do NOT add Sodium Azide!
Storage 4 °C/-20 °C
Storage Comment Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  Expiration date is one (1) year from date of opening.
Expiry Date 12 months
Product cited in: Harper, Guo, Rizzo et al.: "Th17 cytokines stimulate CCL20 expression in keratinocytes in vitro and in vivo: implications for psoriasis pathogenesis." in: The Journal of investigative dermatology, Vol. 129, Issue 9, pp. 2175-83, 2009 (PubMed).

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