PDK-1 (3-Phosphoinositide-Dependant Protein Kinase-1) antibody

Antigen: PDK-1 (3-Phosphoinositide-Dependant Protein Kinase-1)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Cow (Bovine), Mouse (Murine), Rat (Rattus)

Application: Immunohistochemistry (IHC), ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN105431

Additional Information

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 301-312 of PDK-1 (genome data base accession number AC005591). Peptide sequence: R-K-I-Q-E-V-W-R-Q-R-Y-Q-S

Format: Liquid (sterile filtered)

Description: PDK-1 (3-Phosphoinositide-Dependant Protein Kinase-1) phosphorylates AGC kinases. PDK-1 activates conventional PKC and PKCz (zeta) through phosphorylation of critical threonine residues in the activation loop. PDK-1 also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of phosphatidylinositol-3,4,5-trisphosphate. Active Akt inactivates Glycogen Synthase Kinase-3 (GSK3), eventually leading to the dephosphorylation and activation of glycogen synthase, and the stimulation of glycogen synthesis. Because of the role that PDK1 plays in insulin-induced glycogen synthesis and PKC activation, it is a potentially important target for metabolic drug research.

Specificity: This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. This antibody is primarily directed against 60,000 MW human PDK-1 and is useful in determining its presence in various assays. Reactivity with PDK-1 from mouse or other sources has not been determined.

Application Details

Application Notes: Recommended Dilutions: This product has been assayed by immunoblot using HRP Goat-anti-Rabbit IgG [H&L] (code ABIN101990) and Enhanced Chemiluminescence for detection. A working dilution range of 1:750 is suggested to detect PDK-1 in lysates derived from Sf9 insect cells infected with virus expressing myc-tagged human PDK1. In this experiment, ~ 10 ug of both an untransfected sf9 cell lysate (not shown) and PDK-1 virus-infected Sf9 lysate were separated on a 10% gel by SDS-PAGE followed by transfer to nitrocellulose. Primary antibody reaction proceeded overnight at 4

Concentration: 1.3 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.

Research Area: Kinases/Phosphatases

Restrictions: For Research Use only

Images

Immunoblot of Sf9 insect cell lysate. antibodies-online Immunochemical's affinity purified anti-Human PDK-1 detects 60 kDa human PDK-1 in lysates from myc-tagged human PDK-1 virus infected Sf9 cells. A 1:750 dilution is suggested followed by reaction with HRP conjugated Goat-anti-Rabbit IgG [H&L] and chemiluminescent detection. Other detection systems will yield similar results.