Recombinant PDPK1 antibody

Catalog No. ABIN7797423

The Rabbit Monoclonal anti-PDPK1 antibody is suitable to detect PDPK1 in samples from Human. It has been validated for WB and FACS.

Quick Overview for Recombinant PDPK1 antibody (ABIN7797423)

Target: PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This PDPK1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Grade: KD Validated

Clone: 24GB5425

Product Details anti-PDPK1 Antibody

Expression System: HEK-293 Cells

Purpose: KD-Validated PDPK1 Recombinant Rabbit mAb

Cross-Reactivity: Rat

Purification: Affinity Purified

Immunogen: A synthesized peptide derived from human PDPK1

Isotype: IgG

Application Details

Application Notes: Western Blotting (WB): 1:1,000-1:5,000, Flow Cytometry (FCM): 1:200-1:2,000

Comment:

We assess the antibodies’ specificity and selectivity using knockdown techniques. Our validation approach is straightforward: if an antibody identifies a signal in wild-type (WT) cells, but this signal is absent or noticeably reduced in gene-silenced (KD) cells, we consider the antibody to be highly specific.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS (pH 7.4) containing 50% glycerol and 0.02% sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C for one year.

Expiry Date: 12 months

Target Details for PDPK1

Target: PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Alternative Name: PDPK1

Molecular Weight: 63 kDa

Gene ID: 5170

UniProt: O15530

Pathways: PI3K-Akt Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals