Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)
Specificity
At least two isoforms of SPATA4 are known to exist, this antibody will detect both isoforms. SPATA4 antibody is predicted to not cross-react with SPATA protein family members
Purification
Affinity chromatography purified via peptide column
Immunogen
SPATA4 antibody was raised against an AA 18 synthetic peptide near the carboxy terminus of human SPATA4.
SPATA4
Reactivity: Mouse
WB, IF (cc), IF (p)
Host: Rabbit
Polyclonal
AbBy Fluor® 647
Application Notes
SPATA4 antibody can be used for detection of SPATA4 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. Optimal dilutions for each application to be determined by the researcher.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
PBS containing 0.02 % sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage
4 °C/-20 °C
Storage Comment
SPATA4 antibody can be stored at 4 °C for three months and -20 °C, stable for up to one year.
Expiry Date
12 months
Target
SPATA4
(Spermatogenesis Associated Protein 4 (SPATA4))
Synonyms: Spermatogenesis associated protein 4, testis and spermatogenesis cell apoptosis related gene 2, TSARG2, SPEF1B
The spermatogenesis associated protein 4 (SPATA4) protein, also known as TSARG1, is highly expressed in testis, and to a much lower extent in other tissues. SPATA4 contains a highly conserved DUF1042 domain, but no significant homology with any other known protein. SPATA4 is thought to play an important role in the development of cryptorchid testis and is a testis-specific apoptosis candidate oncogene.