alpha Fetoprotein antibody (AA 31-171)
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- Target See all alpha Fetoprotein (AFP) Antibodies
- alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))
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Binding Specificity
- AA 31-171
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This alpha Fetoprotein antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- Purpose
- Polyclonal Antibody to Alpha-Fetoprotein (AFP)
- Specificity
- The antibody is a rabbit polyclonal antibody raised against AFP. It has been selected for its ability to recognize AFP in immunohistochemical staining and western blotting.
- Purification
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
- Immunogen
- Recombinant Alpha-Fetoprotein (AFP) corresdonding to Ile31~Pro171 with N-terminal His Tag
- Isotype
- IgG
- Top Product
- Discover our top product AFP Primary Antibody
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- Application Notes
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Western blotting: 0.5-2 μg/mL
Immunohistochemistry: 5-20 μg/mL
Immunocytochemistry: 5-20 μg/mL
Optimal working dilutions must be determined by end user.
- Comment
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
- Preservative
- ProClin
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- Expiry Date
- 24 months
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Handy, rapid and multiplex detection of tumor markers based on encoded silica-hydrogel hybrid beads array chip." in: Biosensors & bioelectronics, Vol. 48, pp. 153-7, (2013) (PubMed).
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Handy, rapid and multiplex detection of tumor markers based on encoded silica-hydrogel hybrid beads array chip." in: Biosensors & bioelectronics, Vol. 48, pp. 153-7, (2013) (PubMed).
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- Target
- alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))
- Alternative Name
- Alpha-Fetoprotein (AFP Products)
- Synonyms
- FETA antibody, HPAFP antibody, alpha fetoprotein antibody, alpha-fetoprotein antibody, AFP antibody, Afp antibody
- Background
- AFP, A-FP, FETA, HPAFP, Alpha-Fetoglobulin, Alpha-1-fetoprotein
- Pathways
- C21-Steroid Hormone Metabolic Process
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