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DDX20 antibody (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 20) (AA 368-548)

Details for Product anti-DDX20 Antibody No. ABIN108548, Supplier: Log in to see
Antigen
  • DDX20
  • Dp103
  • dp103
  • DP103
  • GEMIN3
  • si:dkey-193l3.3
  • wu:fb16g08
  • wu:fb59a11
Epitope
AA 368-548
19
15
5
4
3
3
3
3
2
2
2
2
1
1
1
1
1
1
Reactivity
Hamster, Human, Mouse (Murine), Rat (Rattus)
102
29
13
1
Host
Mouse
55
49
Clonality (Clone)
Monoclonal ()
Conjugate
This DDX20 antibody is un-conjugated
4
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
94
77
40
18
16
7
6
5
1
Supplier
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Immunogen Recombinant 6His-tag COOH-terminal domain of Gemin3 (amino acids 368-548)
Clone 12H12
Isotype IgG1
Purification Protein A
Alternative Name Gemin 3 (DDX20 Antibody Abstract)
Background Synonyms:
Component of gems 3, DDX 20, DEAD box protein, DEAD-box protein DP103, DEAD/H BOX 20, DKFZP434H052, DP103, SMN interacting protein
Gene ID 11218
OMIM 606168
UniProt Q9UHI6
Research Area Chromatin and Nuclear Signaling, DNA/RNA
Application Notes Optimal antibody dilution should be determined by titration, however as a guideline try: WB: Use at an assay dependent dilution. Detects a band of approximately 105 kDa (predicted molecular weight: 99 kDa). Flow Cyt: Use 1 μg for 106 cells. ICC/IF: 1/50.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Purified antibody (from supernatant) containing PBS 0.1 % sodium azide,
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
 image for anti-DDX20 antibody (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 20) (AA 368-548) (ABIN108548) anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 20 (DDX20) (AA 368-548) antibody
 image for anti-DDX20 antibody (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 20) (AA 368-548) (ABIN108548) anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 20 (DDX20) (AA 368-548) antibody (Image 2)
Product cited in: Mahmoudi, Henriksson, Weibrecht, Smith, Söderberg, Strömblad, Wiman, Farnebo: "WRAP53 is essential for Cajal body formation and for targeting the survival of motor neuron complex to Cajal bodies." in: PLoS biology, Vol. 8, Issue 11, pp. e1000521, 2010 (PubMed).

Bradrick, Gromeier: "Identification of gemin5 as a novel 7-methylguanosine cap-binding protein." in: PLoS ONE, Vol. 4, Issue 9, pp. e7030, 2009 (PubMed).

Castelló, Izquierdo, Welnowska, Carrasco: "RNA nuclear export is blocked by poliovirus 2A protease and is concomitant with nucleoporin cleavage." in: Journal of cell science, Vol. 122, Issue Pt 20, pp. 3799-809, 2009 (PubMed).

Walker, Rajendra, Saieva, Fuentes, Pellizzoni, Matera: "SMN complex localizes to the sarcomeric Z-disc and is a proteolytic target of calpain." in: Human molecular genetics, Vol. 17, Issue 21, pp. 3399-410, 2008 (PubMed).

Morency, Sabra, Catez, Texier, Lomonte: "A novel cell response triggered by interphase centromere structural instability." in: The Journal of cell biology, Vol. 177, Issue 5, pp. 757-68, 2007 (PubMed).

Hamamoto, Nishitsuji, Amagasa, Kannagi, Masuda: "Identification of a novel human immunodeficiency virus type 1 integrase interactor, Gemin2, that facilitates efficient viral cDNA synthesis in vivo." in: Journal of virology, Vol. 80, Issue 12, pp. 5670-7, 2006 (PubMed).

Zhang, Xing, Rossoll, Wichterle, Singer, Bassell: "Multiprotein complexes of the survival of motor neuron protein SMN with Gemins traffic to neuronal processes and growth cones of motor neurons." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 26, Issue 33, pp. 8622-32, 2006 (PubMed).

Charroux, Pellizzoni, Perkinson, Shevchenko, Mann, Dreyfuss: "Gemin3: A novel DEAD box protein that interacts with SMN, the spinal muscular atrophy gene product, and is a component of gems." in: The Journal of cell biology, Vol. 147, Issue 6, pp. 1181-94, 2000 (PubMed).