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DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 20 (DDX20) (AA 368-548) antibody

Details for Product No. ABIN108548, Supplier: Log in to see
Antigen
  • DDX20
  • DP103
  • GEMIN3
  • dp103
  • Dp103
  • si:dkey-193l3.3
  • wu:fb16g08
  • wu:fb59a11
Epitope
AA 368-548
19
15
5
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human
59
12
8
Host
Mouse
46
15
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
2
2
2
2
2
2
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
52
37
15
12
7
2
1
1
1
Supplier
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Immunogen Recombinant 6His-tag COOH-terminal domain of Gemin3 (AA 368-548)
Clone 12H12
Isotype IgG1
Characteristics Myeloma fusion partners:
SP2/0
Detects a band of approximately 105 kDa (predicted molecular weight: 99 kDa).
Purification Protein A
Alternative Name Gemin 3 (DDX20 Antibody Abstract)
Background Synonyms:
Component of gems 3, DDX 20, DEAD box protein, DEAD-box protein DP103, DEAD/H BOX 20, DKFZP434H052, DP103, SMN interacting protein
UniProt Q9UHI6
Research Area Chromatin and Nuclear Signaling, DNA/RNA
Application Notes Optimal antibody dilution should be determined by titration, however as a guideline try: WB: Use at an assay dependent dilution. Flow Cyt: Use 1 µg for 106 cells. ICC/IF: 1/50.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS, 0.1 % sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Storage 4 °C
Supplier Images
 image for anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 20 (DDX20) (AA 368-548) antibody (ABIN108548) anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 20 (DDX20) (AA 368-548) antibody
 image for anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 20 (DDX20) (AA 368-548) antibody (ABIN108548) anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 20 (DDX20) (AA 368-548) antibody (Image 2)
Product cited in: Mahmoudi, Henriksson, Weibrecht et al.: "WRAP53 is essential for Cajal body formation and for targeting the survival of motor neuron complex to Cajal bodies." in: PLoS biology, Vol. 8, Issue 11, pp. e1000521, 2010 (PubMed).

Castelló, Izquierdo, Welnowska et al.: "RNA nuclear export is blocked by poliovirus 2A protease and is concomitant with nucleoporin cleavage." in: Journal of cell science, Vol. 122, Issue Pt 20, pp. 3799-809, 2009 (PubMed).

Bradrick, Gromeier: "Identification of gemin5 as a novel 7-methylguanosine cap-binding protein." in: PLoS ONE, Vol. 4, Issue 9, pp. e7030, 2009 (PubMed).

Walker, Rajendra, Saieva et al.: "SMN complex localizes to the sarcomeric Z-disc and is a proteolytic target of calpain." in: Human molecular genetics, Vol. 17, Issue 21, pp. 3399-410, 2008 (PubMed).

Morency, Sabra, Catez et al.: "A novel cell response triggered by interphase centromere structural instability." in: The Journal of cell biology, Vol. 177, Issue 5, pp. 757-68, 2007 (PubMed).

Zhang, Xing, Rossoll et al.: "Multiprotein complexes of the survival of motor neuron protein SMN with Gemins traffic to neuronal processes and growth cones of motor neurons." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 26, Issue 33, pp. 8622-32, 2006 (PubMed).

Hamamoto, Nishitsuji, Amagasa et al.: "Identification of a novel human immunodeficiency virus type 1 integrase interactor, Gemin2, that facilitates efficient viral cDNA synthesis in vivo." in: Journal of virology, Vol. 80, Issue 12, pp. 5670-7, 2006 (PubMed).

Charroux, Pellizzoni, Perkinson et al.: "Gemin3: A novel DEAD box protein that interacts with SMN, the spinal muscular atrophy gene product, and is a component of gems." in: The Journal of cell biology, Vol. 147, Issue 6, pp. 1181-94, 2000 (PubMed).