ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
Specificity
This is a defatted and defibrinated antiserum.
Purification
Delipidation and Defibrination
Immunogen
This antiserum was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 46-56 of bacterial chloramphenicol acetyl transferase (CAT). This antibody identifies recombinant CAT as a predominant band of 26 kD in eukaryotic cells transfected with a plasmid bearing the CAT gene.
Application Notes
ELISA, IHC, WB (1:500 - 1:2000)
Restrictions
For Research Use only
Format
Liquid
Reconstitution
deionized water
Buffer
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
avoid freeze thaw cycles.
Storage
4 °C/-20 °C
Storage Comment
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.