Goat anti-Mouse IgM (Heavy Chain) Antibody

Catalog No. ABIN1102423

Product Details

Target: IgM

Fragment: F(ab')2 fragment

Binding Specificity: Heavy Chain

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Application: ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB)

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgM and Mouse Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1 % cross-reactivity against other mouse heavy or light chain isotypes.

Purification: Immunoaffinity purified

Sterility: Sterile filtered

Immunogen: Anti-Mouse IgM was produced by repeated immunization with Mouse IgM heavy chain in goat.

Type of Immunogen: Purified protein

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, Flo, IHC (1:1000 - 1:5000), WB (1:200 - 1:2000)

Usage: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10 x10⁶ in flow cytometry is approximately 1.0 μg of antibody. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide, sterile filtered

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details

Target: IgM

Abstract: IgM Products

Target Type: Antibody