NEDD1 antibody

Catalog No. ABIN1103223

Product Details anti-NEDD1 Antibody

Target: NEDD1 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 1 (NEDD1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEDD1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: This antibody reacts with endogenous Nedd1 protein. A BLAST analysis was used to suggest reactivity with Nedd1 from human, chimpanzee, macaque, marmoset, cattle, rat, and mouse based on a 100 % homology with the immunizing sequence. Expect partial reactivity with Nedd1 from turkey, chicken, salmon, and Danio based on a 91 % homology with the immunizing sequence. Cross-reactivity with Nedd1 from other sources has not been determined.

Purification: Immunoaffinity purified

Sterility: Sterile filtered

Immunogen: Anti-NEDD1 was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to the 343-667 region of human Nedd1.

Type of Immunogen: Recombinant protein

Application Details

Application Notes: Approved: ELISA, WB (1:10000)

Usage: This antiserum has been tested for use in ELISA and western blotting using a recombinant truncated Nedd1 protein. Specific conditions for reactivity and detection of Nedd1 should be optimized by the end user. Expect a band approximately ~73 kD in size corresponding to Nedd1 by western blotting in the appropriate cell lysate or extract.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide, sterile filtered

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for NEDD1

Target: NEDD1 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 1 (NEDD1))

Alternative Name: NEDD1 (NEDD1 Products)

Synonyms: MGC81767 antibody, MGC145237 antibody, GCP-WD antibody, TUBGCP7 antibody, neural precursor cell expressed, developmentally down-regulated 1 L homeolog antibody, neural precursor cell expressed, developmentally down-regulated 1 antibody, cilia and flagella associated protein 54 antibody, neural precursor cell expressed, developmentally down-regulated gene 1 antibody, nedd1.L antibody, nedd1 antibody, CFAP54 antibody, NEDD1 antibody, Nedd1 antibody

Background: Name/Gene ID: NEDD1

Synonyms: NEDD1, GCP-WD, NEDD-1, TUBGCP7, Protein NEDD1

Gene ID: 121441

UniProt: Q8NHV4

Pathways: M Phase