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Myc Tag antibody

Details for Product No. ABIN1105584, Supplier: Log in to see
Antigen
Reactivity
Tag
217
36
14
11
6
5
3
1
1
Host
Mouse
163
63
17
16
3
2
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
27
23
14
9
5
4
4
4
4
4
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
221
119
77
52
51
34
22
19
19
7
6
5
5
5
5
4
3
3
3
2
1
1
1
1
1
1
1
Options
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Immunogen GST-6myc-Tag fusion protein
Sequence EQKLISEEDL
Clone PL14
Isotype IgG1
Specificity This antibody can be used for epitope-tagging using the amino acid sequence EQKLISEEDL.
Purification Protein-A Sepharose
Alternative Name C-Myc Epitope Tag
Target Type Tag
Background Epitope tagging is a widely accepted technique that fuses an epitope peptide to a protein as a marker for gene expression. With this technique, the gene expression can be easily monitored on Western blotting, immunoprecipitation and immunofluorescence utilizing an antibody that recognizes such an epitope. Amino acid sequences that are widely used for the epitope tagging are as follows, YPYDVPDYA (HA-Tag), EQKLISEEDL (Myc-Tag) and YTDIEMNRLGK (VSV-G-Tag), which correspond to the partial peptide of Influenza hemagglutinin protein, Human c-myc gene product and Vesicular stomatitis virus glycoprotein respectively.Synonyms: c-myc tag, myc tag, myc-tag
Gene ID 4609
NCBI Accession NP_002458
UniProt P01106
Research Area Transcription Factors, Cancer, Cell Cycle
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Buffer PBS containing 50 % glycerol, without preservatives
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store (in aliquots) at -20 °C.
Background publications Heald, McLoughlin, McKeon: "Human wee1 maintains mitotic timing by protecting the nucleus from cytoplasmically activated Cdc2 kinase." in: Cell, Vol. 74, Issue 3, pp. 463-74, 1993 (PubMed).

Field, Nikawa, Broek, MacDonald, Rodgers, Wilson, Lerner, Wigler: "Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method." in: Molecular and cellular biology, Vol. 8, Issue 5, pp. 2159-65, 1988 (PubMed).