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Myc Tag antibody (Biotin)

IP, IHC, ELISA, WB Host: Rabbit Polyclonal Biotin
Catalog No. ABIN104384
$482.90
Plus shipping costs $45.00
100 μg
Shipping to: United States
Delivery in 1 to 2 Business Days
  • Target See all Myc Tag products
    Myc Tag
    Reactivity
    Please inquire
    Host
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    Rabbit
    Clonality
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    Polyclonal
    Conjugate
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    This Myc Tag antibody is conjugated to Biotin
    Application
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    Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
    Sequence
    EQKLISEEDL
    Specificity
    This affinity purified antibody is directed against human c-myc and is useful in determining its presence in various assays. This polyclonal anti-Myc-tag antibody detects overexpressed proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-Rabbit Serum.
    Characteristics
    Concentration Definition: by UV absorbance at 280 nm
    Immunogen
    This antibody was purified from whole rabbit serum prepared by repeated immunizations with Myc epitope tag peptide E-Q-K-L-I-S-E-E-D-L conjugated to KLH using maleimide. The sequence corresponds to aa 410-419 of human c-Myc.
    Isotype
    IgG
  • Application Notes
    Suggested dilutions:
    ELISA : 1:100,000 – 1:500,000
    Western Blot : 1:2,000 - 1:10,000
    Immunohistochemistry: 1:1,000 - 1:5,000
    Other Dilution: User Optimized
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Concentration
    1.0 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C/-20 °C
    Storage Comment
    tore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 ° C as an undiluted liquid. Dilute only prior to immediate use.
  • Target
    Myc Tag
    Abstract
    Myc Tag Products
    Target Type
    Tag
    Background
    Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies.  Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties.   Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence.  This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means.  This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein.  The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures.  Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.  Supplier produces anti-epitope tag antibodies against many common epitope tags including Myc, GST, GFP, 6X His, MBP, FLAG and HA. Supplier also produces antibodies to other tags including FITC, Rhodamine (TRITC), DNP and biotin.
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