Myc Tag antibody (Biotin)

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Antigen
Reactivity
Tag
213
36
14
10
6
5
3
1
1
Host
Mouse
161
60
17
16
3
2
1
Clonality (Clone)
Monoclonal ()
Conjugate
Biotin
27
23
14
9
5
4
4
4
4
4
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
216
116
78
53
48
34
22
19
19
7
6
5
5
5
5
4
3
3
3
2
1
1
1
1
1
1
1
Options
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Immunogen GST-6myc-Tag fusion protein
Sequence EQKLISEEDL
Clone PL14
Isotype IgG1
Specificity This antibody recognizes Myc-Tag peptide sequence (EQKLISEEDL).
Purification Protein-A Sepharose
Alternative Name C-Myc Epitope Tag
Target Type Tag
Background Epitope tagging has widely been accepted technique that fuse an epitope peptide to a certain protein as a marker for gene expression. With this technique, the gene expression can be easily monitored on western blotting, immunoprecipitation and immunofluorescence utilizing with an antibody that recognizes such an epitope. Amino acid sequences that are widely used for the epitope tagging are as follow, YPYDVPDYA (HA-Tag), EQKLISEEDL (Myc-Tag) and YTDIEMNRLGK (VAV-G- Tag), which corresponding to the partial peptide of Influenza hemagglutinin protein, Human c-myc gene product and Vesicular stomatitis virus glycoprotein respectively.Synonyms: c-myc tag, myc tag, myc-tag
Gene ID 4609
NCBI Accession NP_002458
UniProt P01106
Research Area Transcription Factors, Cancer, Cell Cycle
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Buffer NaPB ( pH 8.0), 0.3 M NaCl and 0.09 % NaN3
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store at 2 - 8 °C.
Background publications Heald, McLoughlin, McKeon: "Human wee1 maintains mitotic timing by protecting the nucleus from cytoplasmically activated Cdc2 kinase." in: Cell, Vol. 74, Issue 3, pp. 463-74, 1993 (PubMed).

Field, Nikawa, Broek, MacDonald, Rodgers, Wilson, Lerner, Wigler: "Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method." in: Molecular and cellular biology, Vol. 8, Issue 5, pp. 2159-65, 1988 (PubMed).