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CD5 antibody (CD5 Molecule)

Details for Product anti-CD5 Antibody No. ABIN1106433, Supplier: Log in to see
Antigen
  • CD5
  • LEU1
  • Ly-1
  • Ly-12
  • Ly-A
  • Lyt-1
  • T1
Reactivity
Human
808
234
139
75
56
40
40
36
30
30
29
27
26
22
16
3
1
1
1
Host
Mouse
953
199
124
Clonality (Clone)
Monoclonal ()
Conjugate
This CD5 antibody is un-conjugated
151
131
99
56
30
28
24
22
19
19
18
18
18
18
18
18
18
14
14
10
10
8
6
4
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
1019
401
297
274
223
181
168
122
116
15
12
11
11
8
8
8
5
2
1
1
1
1
1
Supplier
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Immunogen Human acute lymphoblastic leukemia (ALL) T cells
Clone L17F12
Isotype IgG2a
Cross-Reactivity (Details) Species reactivity (tested):Human
Purification Protein-A affinity chromatography
Purity > 95 % pure by SDS-PAGE
Alternative Name CD5 (CD5 Antibody Abstract)
Background CD5 antigen (T1, 67 kDa) is a human cell surface T-lymphocyte single-chain transmembrane glycoprotein. CD5 is expressed on all mature T-lymphocytes, most of thymocytes, subset of B-lymphocytes and on many T-cell leukemias and lymphomas. It is a type I membrane glycoprotein whose extracellular region contains three scavenger receptor cysteine-rich (SRCR) domains. The CD5 is a signal transducing molecule whose cytoplasmic tail is devoid of any intrinsic catalytic activity. CD5 modulates signaling through the antigen-specific receptor complex (TCR and BCR). CD5 crosslinking induces extracellular Ca++ mobilization, tyrosine phosphorylation of intracellular proteins and DAG production. Preliminary evidence shows protein associations with ZAP-70, p56lck, p59fyn, PC-PLC, etc. CD5 may serve as a dual receptor, giving either stimulatory or inhibitory signals depending both on the cell type and development stage. In thymocytes and B1a cells seems to provide inhibitory signals, in peripheral mature T lymhocytes it acts as a costimulatory signal receptor. CD5 is the phenotypic marker of a B cell subpopulation involved in the production of autoreactive antibodies. Disease relevance: CD5 is a phenotypic marker for some B cell lymphoproliferative disorders (B-CLL, Hairy cell leukemia, etc.). The CD5+ popuation is expanded in some autoimmune disorders (Rheumatoid Arthritis, etc.). Herpes virus infections induce loss of CD5 expression in the expanded CD8+ human T cells.Synonyms: CD5, LEU1, Lymphocyte antigen T1/Leu-1, T-cell surface glycoprotein CD5
Gene ID 921
NCBI Accession NP_055022
UniProt P06127
Research Area Stem Cells, Hematopoietic Progenitors, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline (PBS), approx. pH 7.4, 15 mM sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Dunphy, Tang: "The value of CD64 expression in distinguishing acute myeloid leukemia with monocytic differentiation from other subtypes of acute myeloid leukemia: a flow cytometric analysis of 64 cases." in: Archives of pathology & laboratory medicine, Vol. 131, Issue 5, pp. 748-54, 2007

Gong, Lagoo, Peters, Horvatinovich, Benz, Buckley: "Value of CD23 determination by flow cytometry in differentiating mantle cell lymphoma from chronic lymphocytic leukemia/small lymphocytic lymphoma." in: American journal of clinical pathology, Vol. 116, Issue 6, pp. 893-7, 2001

McAlister, Davis, Pfuhl, Driscoll: "NMR analysis of the N-terminal SRCR domain of human CD5: engineering of a glycoprotein for superior characteristics in NMR experiments." in: Protein engineering, Vol. 11, Issue 10, pp. 847-53, 1999

Shuster, Falletta, Pullen, Crist, Humphrey, Dowell, Wharam, Borowitz: "Prognostic factors in childhood T-cell acute lymphoblastic leukemia: a Pediatric Oncology Group study." in: Blood, Vol. 75, Issue 1, pp. 166-73, 1990

Engleman, Warnke, Fox, Dilley, Benike, Levy: "Studies of a human T lymphocyte antigen recognized by a monoclonal antibody." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 78, Issue 3, pp. 1791-5, 1981