Cyclin B1 antibody

Catalog No. ABIN1106867

Product Details anti-Cyclin B1 Antibody

Target: Cyclin B1 (CCNB1)

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cyclin B1 antibody is un-conjugated

Application: Immunoprecipitation (IP)

Specificity: This antibody immunoprecipitates Cyclin B1 (55 kDa).

Purification: Protein-A Agarose Chromatography of hybridoma supernatant.

Immunogen: Hamster recombinant Cyclin B1.

Clone: V92-1

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.2 containing 50 % Glycerol without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for Cyclin B1

Target: Cyclin B1 (CCNB1)

Alternative Name: Cyclin B1 (CCNB1 Products)

Synonyms: ccnb antibody, cycb antibody, cb267 antibody, cycb1 antibody, wu:fa19g04 antibody, wu:fb16d01 antibody, wu:fb16e07 antibody, wu:fi21c01 antibody, ccnb1 antibody, MGC53596 antibody, CCNB antibody, Ccnb1-rs1 antibody, Ccnb1-rs13 antibody, CycB1 antibody, Cycb-4 antibody, Cycb-5 antibody, Cycb1-rs1 antibody, cyclin B1 antibody, cyclin B1 S homeolog antibody, ccnb1 antibody, ccnb1.2 antibody, ccnb1.S antibody, CCNB1 antibody, Ccnb1 antibody, ccnb1.2.S antibody

Background: Cyclins are evolutionarily conserved regulatory proteins that are essential for controlling the progression of the cell-cycle. Proliferating cells express both cyclin B1 and cyclin B2, expression is normally low in G0/G1, increases in S, and is maximal during the G2/M phase of cell division. Cyclin B1 can be found both on intracellular membranes and free in the cytoplasm, in contrast to cyclin B2, which is membrane-associated. Cyclin B1 binds to p34Cdc2, also known as Cdk1, a cyclin-dependent kinase, to form an inactive complex that accumulates in the cytoplasm. Dephosphorylation by Cdc25 results in activation of the complex and translocation to the nucleus. Cyclin B1 is rapidly degraded at the end of mitosis, and this degradation is essential for cells to exit from mitosis.Synonyms: CCNB, CCNB1, Cyclin-B1, G2/mitotic-specific cyclin-B1

Gene ID: 891

NCBI Accession: NP_114172

UniProt: P14635

Pathways: Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase