HA-Tag antibody

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Antigen
Reactivity
Tag
454
31
18
2
1
1
1
Host
Rabbit
210
181
40
34
20
3
Clonality
Polyclonal
Conjugate
Un-conjugated
45
31
24
10
10
9
8
8
7
7
7
7
6
6
6
6
6
4
4
4
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
390
201
121
117
84
81
68
40
15
13
11
7
7
5
4
4
4
4
3
2
2
2
1
1
1
1
1
Options
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Immunogen A synthetic peptide from influenza hemagglutinin epitope coupled to KLH
Sequence YPYDVPDYA
Isotype IgG
Cross-Reactivity (Details) Species reactivity (tested):Mammalia
Purification Affinity chromatography
Alternative Name HA Epitope Tag (YPYDVPDYA)
Target Type Tag
Background Plasmid vectors for the expression of coding regions of eukaryotic genes in acterial, insect and mammalian hosts are in common usage, such expression ectors are frequently used to encode hybrid fusion proteins consisting of a eukaryotic target protein and a specialized region designed to aid in the urification and visualization of the target protein. For example, the pCDM8 expression vector and derivatives thereof encode fusions between the target protein and an 11 amino acid peptide derived from the influenza protein hemagglutinin (HA).Synonyms: HA Tag, HA-Tag, Hemagglutinin Tag
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4,with 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
Background publications Field, Nikawa, Broek, MacDonald, Rodgers, Wilson, Lerner, Wigler: "Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method." in: Molecular and cellular biology, Vol. 8, Issue 5, pp. 2159-65, 1988 (PubMed).

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