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HA-Tag antibody

Details for Product No. ABIN1107476, Supplier: Log in to see
Antigen
Reactivity
Tag
381
16
11
2
2
2
1
1
Host
Rabbit
161
158
37
34
20
3
Clonality
Polyclonal
Conjugate
Un-conjugated
41
31
26
8
5
4
4
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
332
191
116
83
55
40
40
18
12
11
9
7
5
5
5
4
3
2
2
2
1
1
1
Supplier
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Immunogen A synthetic peptide from influenza hemagglutinin epitope coupled to KLH
Sequence YPYDVPDYA
Isotype IgG
Cross-Reactivity (Details) Species reactivity (tested):Mammalia
Purification Affinity chromatography
Alternative Name HA Epitope Tag (YPYDVPDYA)
Target Type Tag
Background Plasmid vectors for the expression of coding regions of eukaryotic genes in acterial, insect and mammalian hosts are in common usage, such expression ectors are frequently used to encode hybrid fusion proteins consisting of a eukaryotic target protein and a specialized region designed to aid in the urification and visualization of the target protein. For example, the pCDM8 expression vector and derivatives thereof encode fusions between the target protein and an 11 amino acid peptide derived from the influenza protein hemagglutinin (HA).Synonyms: HA Tag, HA-Tag, Hemagglutinin Tag
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4,with 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
Background publications Field, Nikawa, Broek, MacDonald, Rodgers, Wilson, Lerner, Wigler: "Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method." in: Molecular and cellular biology, Vol. 8, Issue 5, pp. 2159-65, 1988 (PubMed).