PIWIL1 antibody (Internal Region)

Catalog No. ABIN1108654

Product Details anti-PIWIL1 Antibody

Target: PIWIL1 (Piwi-Like 1 (PIWIL1))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat, Dog, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This PIWIL1 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequence: C-SNRKDKYDAI KKY

Specificity: This antibody is expected to recognise both reported isoforms (NP_004755.2, NP_001177900.1).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog), Porcine.
Species reactivity (tested):Human.

Purification: Affinity Chromatography

Immunogen: Synthetic peptide (C-SNRKDKYDAIKKY) from the internal region of the protein sequence according to NP_004755.2, NP_001177900.1. Genename: PIWIL1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PIWIL1

Target: PIWIL1 (Piwi-Like 1 (PIWIL1))

Alternative Name: PIWIL1 (PIWIL1 Products)

Synonyms: ziwi antibody, PIWI antibody, HIWI antibody, MIWI antibody, piwi like RNA-mediated gene silencing 1 antibody, PIWI-like protein 1 antibody, putative PIWI-like protein 1 antibody, putative argonaut-like protein antibody, piwi-like RNA-mediated gene silencing 1 antibody, PIWIL1 antibody, Tb10.70.5520 antibody, LINJ_21_0470 antibody, LBRM_21_0470 antibody, piwil1 antibody, Piwil1 antibody

Background: PIWI-L1 is a member of the PIWI subfamily of Argonaute proteins, evolutionarily conserved proteins containing both PAZ and Piwi motifs that are crucial for the biogenesis and function of small non-coding RNAs and play important roles in stem cell self-renewal, RNA silencing, and translational regulation in diverse organisms. PIWI-L1 is thought to play a role as an intrinsic regulator of the self-renewal capacity of germline and hematopoietic stem cells as removing PIWI-L1 protein from single germline stem cells significantly decreases the rate of their division. Recent studies have shown that overexpression of PIWI-L1 (as well as other PIWI-like proteins) are potential biomarkers for colon and other cancers.Synonyms: HIWI, Piwi-like protein 1

Gene ID: 9271

NCBI Accession: NP_001177900