Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Specificity
This antibody detects ROCK2 (N-term).
Cross-Reactivity (Details)
Species reactivity (expected):MouseSpecies reactivity (tested):Human, rat
Purification
Immunogen affinity purified
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminal of human ROCK2 .Remarks: Sequence is identical to the related rat and mouse sequence.
This product contains Sodium azide and Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the lyophilized product at -20 °C. Following reconstitution, the antibody can be stores at 2-8 °C for up to one month or (in aliquots) at -20 °C for longer.
Target
ROCK2
(rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2))
Rho-associated kinase (ROCK), including the ROCK-I and ROCK-II isoforms, is a protein kinase involved in signaling from Rho to actin cytoskeleton. Serine/threonine kinase ROCK II/Rho kinase, which is an isozyme of ROCK I, is one of the targets for the small GTPase Rho. ROCK II regulates the formation of actin stress fibers and focal adhesions, cytokinesis, smooth muscle contraction, and the activation of c-fos serum response element. Sequencing analysis has shown that human ROCK II contains 1388 amino acid residues with a calculated molecular mass of approximately 161 kDa. Fluorescence in situ hybridization analysis showed that the human ROCK II gene is located on chromosome 2p24.1 Thumkeo et al. (2003) concluded that ROCK-II is essential in inhibiting blood coagulation and maintaining blood flow in the endothelium-free labyrinth layer and that loss of ROCK-II leads to thrombus formation, placental dysfunction, intrauterine growth retardation, and fetal death.2Synonyms: KIAA0619, ROCK-2, Rho kinase 2, Rho-associated, Rho-associated protein kinase 2, coiled-coil-containing protein kinase 2, p164ROCK-2