TOP2A / TOP2B (N-Term) antibody

Details for Product No. ABIN1109282
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Antigen
Epitope
N-Term
Reactivity
Human, Mouse (Murine), Rat (Rattus)
Host
Mouse
Clonality (Clone)
Monoclonal ()
Application
Western Blotting (WB)
Pubmed 8 references available
Quantity 0.1 mg
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Catalog No. ABIN1109282
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Immunogen Recombinant N-terminal Topoisomerase II fusion protein
Clone AK5
Isotype IgG1
Specificity This antibody reacts with Topo IIa and ß. The antibody detects 170 KD of human Topo IIa and 180 KD of human Topo IIß on Western blot with whole cell lysate.
Purification Protein-A Sepharose
Background Eukaryotic DNA Topoisomerase II is a ubiquitous ATP-dependent type II Topoisomerase that play an important role in DNA replication, transcription and recombination by changing the degree of superhelicity, catenation and knotting of topologically closed DNA molecules. Two isoforms of DNA Topoisomerases II have been reported in mammalian cells, Topoisomerase IIa and IIß. cDNA sequence of those isoforms reveals that both type II Topoisomerases are structurally and catalytically close. However, Topoisomerase IIß is expressed with no cell cycle dependence, whereas the expression of Topoisomerase IIa is cell cycle-regulated, peaking in G2-M phase and declining to a minimal level at the end of M phase. Topoisomerase IIa is poly-ubiquitinated and degraded preferentially in the apoptosis-induced human epithermoid carcinoma-derived cell line, MA1, in an ATP-and- ubiquitin-dependent manner. Recent studies also indicated that this isoform is selectively cleaved during Fas-mediated T cell apoptosis and Fas-, Staurosporine- and Anisomycin- mediated Jurkat cell apoptosis.
Alternate names: DNA topoisomerase 2-alpha, DNA topoisomerase 2-beta, DNA topoisomerase II alpha isozyme, DNA topoisomerase II beta isozyme, TOP2, Top-2
Gene ID 7153
NCBI Accession NP_001058.2
UniProt P11388
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS containing 50% glycerol, pH 7.2, without preservatives
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store (in aliquots) at -20 °C.
Expiry Date 12 months
Supplier Images
anti-TOP2A / TOP2B (N-Term) antibody anti-TOP2A / TOP2B (N-Term) antibody
Background publications Tsai-Pflugfelder, Liu, Liu et al.: "Cloning and sequencing of cDNA encoding human DNA topoisomerase II and localization of the gene to chromosome region 17q21-22." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 85, Issue 19, pp. 7177-81, 1988 (PubMed).

Tsutsui, Tsutsui, Okada et al.: "Molecular cloning of partial cDNAs for rat DNA topoisomerase II isoforms and their differential expression in brain development." in: The Journal of biological chemistry, Vol. 268, Issue 25, pp. 19076-83, 1993 (PubMed).

Casiano, Martin, Green et al.: "Selective cleavage of nuclear autoantigens during CD95 (Fas/APO-1)-mediated T cell apoptosis." in: The Journal of experimental medicine, Vol. 184, Issue 2, pp. 765-70, 1996 (PubMed).

Nakajima, Morita, Ohi et al.: "Degradation of topoisomerase IIalpha during adenovirus E1A-induced apoptosis is mediated by the activation of the ubiquitin proteolysis system." in: The Journal of biological chemistry, Vol. 271, Issue 40, pp. 24842-9, 1996 (PubMed).

Keeney, Giroux, Kleckner: "Meiosis-specific DNA double-strand breaks are catalyzed by Spo11, a member of a widely conserved protein family." in: Cell, Vol. 88, Issue 3, pp. 375-84, 1997 (PubMed).

Bergerat, de Massy, Gadelle et al.: "An atypical topoisomerase II from Archaea with implications for meiotic recombination." in: Nature, Vol. 386, Issue 6623, pp. 414-7, 1997 (PubMed).

Takemoto, Koike, Mori et al.: "Human herpesvirus 6 open reading frame U14 protein and cellular p53 interact with each other and are contained in the virion." in: Journal of virology, Vol. 79, Issue 20, pp. 13037-46, 2005 (PubMed).

Yoshida, Yamaguchi, Shinagawa et al.: "Protein kinase C delta activates topoisomerase IIalpha to induce apoptotic cell death in response to DNA damage." in: Molecular and cellular biology, Vol. 26, Issue 9, pp. 3414-31, 2006 (PubMed).

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