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Keratin 20 (KRT20) antibody

Details for Product No. ABIN111168, Supplier: Log in to see
Antigen
  • 9030623C06Rik
  • CD20
  • CK-20
  • CK20
  • K20
  • KRT20
  • KRT21
  • Krt21
Reactivity
Human, Pig (Porcine)
235
51
42
7
4
2
1
Host
Mouse
119
104
18
2
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
13
8
5
3
3
3
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
166
82
70
62
24
23
13
11
9
8
4
4
3
2
1
1
Supplier
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Immunogen Electrophoretically purified keratin K20 from human intestinal mucosa.
Clone KS20-3
Isotype IgG1
Specificity IT-Ks 20.3 represents an excellent marker for certain types of carcinomas such as adenocarcinomas of the colon, transitional cell carcinomas of the bladder and Merkel cell tumors of the skin. Very sensitive detection of intestestinal and gastric foveolar epithelium, urothelial umbrella cells, Merkel cells of epidermis as well as tumors originating therefrom (e.g. primary and metastatic colorectal carcinoma). Adenocarcinomas of breast, lung, endometrium and ovary (non-mucinous) as well as neuroendocrine tumors of the lung are essentially negative. Polypeptide recognized: protein IT (keratin K20, Mr 46 000, formerly also designated cytokeratin 20).
Purification Protein A affinity chromatography
Alternative Name Cytokeratin 20 (KRT20 Antibody Abstract)
Background Cytokeratin 20 is a type I keratin which is primarily expressed in gastric and intestinal epithelium, urothelium, and Merkel-cells. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Their genes are clustered in a region of chromosome 17q12-q21. Cytokeratin 20 is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. It is also expressed in adenocarcinomas of the colon, stomach, pancreas and the bile system and is present in mucinous ovarian tumors, transitional-cell and Merkel-cell carcinomas. Notably, the squamous cell carcinomas and adenocarcinomas of the breast, lung, and endometrium, non-mucinous tumors of the ovary, and small cell carcinomas lack cytokeratin 20.Synonyms: CK20, Cytokeratin 20, K20, KRT20, Keratin type I cytoskeletal 20, Keratin-20, Protein IT
Gene ID 54474
NCBI Accession NP_061883
UniProt P35900
Research Area Signaling, Cytoskeleton, Cytokeratins, Extracellular Matrix, Cancer
Application Notes Immunohistochemistry on frozen sections: 1: 10. Western Blot. Incubation Time: 1 h at RT or over night at 2-8C.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Restore in 1 mL dist. water
Buffer Contains 0.09 % sodium azide, 0.5 % BSA in PBS buffer, pH 7.4
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
 image for anti-Keratin 20 (KRT20) antibody (ABIN111168) References
Background publications Harnden, Eardley, Joyce, Southgate: "Cytokeratin 20 as an objective marker of urothelial dysplasia." in: British journal of urology, Vol. 78, Issue 6, pp. 870-5, 1997

Zhang, Oakley: "The distribution and origin of keratin 20-containing taste buds in rat and human." in: Differentiation; research in biological diversity, Vol. 61, Issue 2, pp. 121-7, 1997

Demirkesen, Hoede, Moll: "Epithelial markers and differentiation in adnexal neoplasms of the skin: an immunohistochemical study including individual cytokeratins." in: Journal of cutaneous pathology, Vol. 22, Issue 6, pp. 518-35, 1996

Möbus, Moll, Gerharz, Kieback, Weikel, Hoffmann, Kreienberg: "Establishment of new ovarian and colon carcinoma cell lines: differentiation is only possible by cytokeratin analysis." in: British journal of cancer, Vol. 69, Issue 3, pp. 422-8, 1994

Moll, Zimbelmann, Goldschmidt, Keith, Laufer, Kasper, Koch, Franke: "The human gene encoding cytokeratin 20 and its expression during fetal development and in gastrointestinal carcinomas." in: Differentiation; research in biological diversity, Vol. 53, Issue 2, pp. 75-93, 1993

Moll, Löwe, Laufer, Franke: "Cytokeratin 20 in human carcinomas. A new histodiagnostic marker detected by monoclonal antibodies." in: The American journal of pathology, Vol. 140, Issue 2, pp. 427-47, 1992

Moll, Moll: "Comparative cytokeratin analysis of sweat gland ducts and eccrine poromas." in: Archives of dermatological research, Vol. 283, Issue 5, pp. 300-9, 1991

Moll, Schiller, Franke: "Identification of protein IT of the intestinal cytoskeleton as a novel type I cytokeratin with unusual properties and expression patterns." in: The Journal of cell biology, Vol. 111, Issue 2, pp. 567-80, 1990