IT-Ks 20.10 represents an excellent marker for certain types of carcinomas such as adenocarcinomas of the colon, transitional cell carcinomas of the bladder and Merkel cell tumors of the skin. Very sensitive detection of intestestinal and gastric foveolar epithelium, urothelial umbrella cells, Merkel cells of epidermis as well as tumors originating therefrom (e.g. primary and metastatic colorectal carcinoma). Adenocarcinomas of breast, lung, endometrium and ovary (non-mucinous) as well as neuroendocrine tumors of the lung are essentially negative. Polypeptide recognized: protein IT (keratin K20, Mr 46 000, formerly also designated cytokeratin 20). Reactivities on cultured cell lines (tested so far): HT-29, LoVo, DLD-1, SW 1116, CaCo-2, RT-4.
Purification
Protein A affinity chromatography
Immunogen
Electrophoretically purified Keratin K20 from human intestinal mucosa
KRT20
Reactivity: Human
IHC, StM
Host: Mouse
Monoclonal
KRT20-3145
unconjugated
Application Notes
Immunohistochemistry on frozen tissue. Immunohistochemistry on paraffin embedded sections (only after after microwavetreatment). Dilute 1/10 with PBS for immunohistochemistry. Incubation time: 1h at RT, orover night at 4C. Western blotting. ELISA. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore with 1 mL distilled water.
Buffer
Final solution contains 0.09 % NaN3, 0.5 % BSA in PBS buffer, pH 7.4.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
-20 °C
de Wit, van Dalum, Lenferink, Tibbe, Hiltermann, Groen, van Rijn, Terstappen: "The detection of EpCAM(+) and EpCAM(-) circulating tumor cells." in: Scientific reports, Vol. 5, pp. 12270, (2015) (PubMed).
Sharma, Cantz, Richter, Eckert, Henschler, Wilkens, Jochheim-Richter, Arseniev, Ott: "Human cord blood stem cells generate human cytokeratin 18-negative hepatocyte-like cells in injured mouse liver." in: The American journal of pathology, Vol. 167, Issue 2, pp. 555-64, (2005) (PubMed).
Cytokeratin 20 is a type I keratin which is primarily expressed in gastric and intestinal epithelium, urothelium, and Merkel-cells. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Their genes are clustered in a region of chromosome 17q12-q21. Cytokeratin 20 is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. It is also expressed in adenocarcinomas of the colon, stomach, pancreas and the bile system and is present in mucinous ovarian tumors, transitional-cell and Merkel-cell carcinomas. Notably, the squamous cell carcinomas and adenocarcinomas of the breast, lung, and endometrium, non-mucinous tumors of the ovary, and small cell carcinomas lack cytokeratin 20.Synonyms: CK20, Cytokeratin 20, K20, KRT20, Keratin type I cytoskeletal 20, Keratin-20, Protein IT