Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

CD42a antibody (FITC)

GP9 Reactivity: Human FACS, IF Host: Mouse Monoclonal GR-P FITC
Catalog No. ABIN1112139
  • Target See all CD42a (GP9) Antibodies
    CD42a (GP9) (Glycoprotein IX (Platelet) (GP9))
    Reactivity
    • 56
    • 12
    • 6
    • 5
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Human
    Host
    • 40
    • 19
    Mouse
    Clonality
    • 42
    • 17
    Monoclonal
    Conjugate
    • 24
    • 8
    • 6
    • 5
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CD42a antibody is conjugated to FITC
    Application
    • 26
    • 21
    • 19
    • 13
    • 12
    • 6
    • 6
    • 2
    • 2
    • 1
    • 1
    Flow Cytometry (FACS), Immunofluorescence (IF)
    Characteristics
    Monoclonal Mouse Anti-Human CD42a, is recommended for use in flow cytometry for identification of Platelets and Megakaryocytes.
    Clone
    GR-P
    Isotype
    IgG2a
    Top Product
    Discover our top product GP9 Primary Antibody
  • Application Notes
    It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 20 µl/10^6 cells.
    Comment

    Fluorescein isothiocyanate (Molecular Probes)

    Assay Procedure

    Procedure for preparation of PLATELETS: 1. Centrifuge tube of freshly drawn EDTA blood (600rpm or 75xg, 20') 2. Remove platelets (top layer) and wash twice over in PBS and resuspend in PBS. 3. Add 20 µl of CD42a FITC and mix gently with a vortex mixer. The 20 µl is a guideline only, the optimal volume should be determined by the individual laboratory. 4. The recommended negative control is a non-reactive PE-conjugated antibody of the same isotype. 5. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 6. Analyse on a flow cytometer.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    The conjugate is provided in liquid form in buffer containing 1% bovine serum albumin (BSA) and 0,09% Sodium azide, pH 7.2.
    Preservative
    Sodium azide
    Precaution of Use
    1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.
    Storage
    4 °C
  • Target
    CD42a (GP9) (Glycoprotein IX (Platelet) (GP9))
    Alternative Name
    CD42a (GP9 Products)
    Synonyms
    cd42a antibody, gpix antibody, GP9 antibody, GPIX antibody, Cd42 antibody, CD42a antibody, glycoprotein IX platelet antibody, glycoprotein IX platelet L homeolog antibody, glycoprotein IX (platelet) antibody, glycoprotein 9 (platelet) antibody, GP9 antibody, gp9.L antibody, Gp9 antibody
    Background
    Platelet glycoprotein IX (GP9) is a small membrane glycoprotein found on the surface of human platelets. It forms a 1-to-1 noncovalent complex with glycoprotein Ib (GP Ib), a platelet surface membrane glycoprotein complex that functions as a receptor for von Willebrand factor (VWF). The main portion of the receptor is a heterodimer composed of 2 polypeptide chains, an alpha chain (GP1BA) and a beta chain (GP1BB), that are linked by disulfide bonds. The complete receptor complex includes noncovalent association of the alpha and beta subunits with GP9 and platelet glycoprotein V (GP5)
You are here:
Support