CD9 antibody (APC)

Catalog No. ABIN1112241

Product Details anti-CD9 Antibody (APC)

Target: CD9

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD9 antibody is conjugated to APC

Application: Flow Cytometry (FACS)

Characteristics: Monoclonal Mouse Anti-Human CD9A is recommended for use in flow cytometry for identification of human platelets.

Purification: Affinity chromatography.

Clone: VJ1-20

Isotype: IgG2a

Application Details

Comment:

Fluorescein isothiocyanate (Molecular Probes)

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The conjugate is provided in liquid form in buffer containing 1% bovine serum albumin (BSA) and 0,09% Sodium azide, pH 7.2.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-CD9 antibody (APC) (ABIN1112241)

Vidal-Gil, Sancho-Pelluz, Zrenner, Oltra, Sahaboglu: "Poly ADP ribosylation and extracellular vesicle activity in rod photoreceptor degeneration." in: Scientific reports, Vol. 9, Issue 1, pp. 3758, (2019) (PubMed).

Target Details for CD9

Target: CD9

Alternative Name: CD9 (CD9 Products)

Synonyms: BTCC-1 antibody, DRAP-27 antibody, MIC3 antibody, MRP-1 antibody, TSPAN-29 antibody, TSPAN29 antibody, Tspan29 antibody, cd9 antibody, wu:fb61g04 antibody, zgc:73383 antibody, zgc:85743 antibody, CD9 molecule antibody, CD9 antigen antibody, CD9 molecule a antibody, CD9 antibody, Cd9 antibody, cd9a antibody

Background: This antibody reacts with a 25 kDa molecular weight. The antigen is expressed on eosinophils, granulocytes, monocytes and numerous stromal cells. This CD9 antigen belongs to the TM4 superfamily of cell surface proteins with the N- and C-terminals cytoplasmically located. This antibody reacts immunohistochemically on frozen sections of platelets, monocytes and pre-B cells with differential staining on lymphoid and epithelial tissues. CLINICAL RELEVANCE - CD9 mAbs inhibit infection by FIV and CDV (canine distemper virus) but CD9 does not directly bind the virus, suggesting a role as a viral co-receptor, as for diphtheria toxin. - CD9 mAbs have been used for immunophenotyping leukemias and bone marrow purging in autologous bone marrow transplantation - The expression of CD9 is inversely correlated with metastasis in melanoma and breast cancer - CD9 expression is inversely correlated with actuarial survival in lung cancer Higashiyama et al. Cancer Research 55: 6040-6044 1995 - Transfection of CD9 in murine melanoma cells has been shown to reduce metastasis PRINCIPLES OF THE TEST. Immunostep CD9: APC monoclonal antibodies bind to the surface of cells that express the CD9 antigen. To identify these cells, peripheral blood leucocytes are incubated with the antibodies and red blood cells are lysed before washing to remove unbound antibodies. An appropriate fixative solution is added to lysed, washed cells before the stained and fixed cells are analysed by flow cytometry with an argon ion laser at 488nm. REAGENT COMPOSITION. Cluster Designation: CD9

Pathways: Response to Water Deprivation, Cell-Cell Junction Organization