Microtubule Associated Protein 2 (MAP2) antibody (Biotin)

Antigen: Microtubule Associated Protein 2 (MAP2)

Synonyms: MAP2A, MAP2B, MAP2C, DKFZp686I2148, MAP2, MAP-2, Mtap-2, xmap2, MGC53131, a730034c02, map2, MGC145864

Clonality: Monoclonal (AP20)

Host: Mouse

Reactivity: Cow (Bovine), Chicken, Human, Mouse (Murine), Rat (Rattus), Xenopus laevis

Conjugate: Biotin

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN111292

Additional Information

Characteristics: Synonyms: Microtubule-associated protein 2, MAP2, Neuronal Marker

Alternative name: MAP-2

Gene ID: 4133

Swiss-Prot: P11137

Immunogen: Bovine brain microtubule associated protein 2.

Isotype: IgG1

Clone: AP20

Description: Microtubule Associated Protein 2 exists in two high molecular weight forms (2a and 2b)and a low molecular weight form (2c). The expression of MAP2 is developmentallyregulated and its multiple forms arise by alternative splicing of a single gene.

Specificity: Anti-MAP-2 recognizes (Mr 300 kDa)MAP-2 protein from bovine brain. The antibodystrongly cross-reacts with MAP-2 from human, rat, mouse, Xenopus, quail and chickenbrains. Anti-MAP-2 (Clone AP20) reacts with the high molecular weight forms (2a & 2b) ofMAP-2 but not with the low molecular weight form (2c). Anti-MAP-2 reacts with dendritesand cell bodies of neurons.

Application Details

Application Notes: Immunohistochemistry on frozen sections: 1/500. Immunofluorescence: 1/500. WesternBlotting: 1-2 ug/ml for 2hrs at RT). Immunohistology (Formalin/paraffin): 1-2 ug/ml for 30min at RT. [Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mMcitrate buffer, pH 6.0, for 10-20 min. followed by cooling at RT for 20 min. ]Anti-MAP-2 may be used in studies of neuron structure in both normal and malignant braintissue. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.1 mg/ml

Buffer: 0.01 M phosphate buffered saline (PBS) pH 7.2, containing, 1.0% BSA and0.09% sodium azide as a preservative.Label: Biotin

Storage: Store the antibody at 2-8°C. Do not freeze. Shelf life: one year from despatch.

Research Area: Cytoskeleton

Restrictions: For Research Use only

Publications

Publications: Lewis, Ivanov, Lee et al.: "Organization of microtubules in dendrites and axons is determined by a short hydrophobic zipper in microtubule-associated proteins MAP2 and tau." in: Nature, Vol. 342, Issue 6249, pp. 498-505, 1990 (PubMed).

Peng, Binder, Black: "Biochemical and immunological analyses of cytoskeletal domains of neurons." in: The Journal of cell biology, Vol. 102, Issue 1, pp. 252-62, 1986 (PubMed).