Microtubule Associated Protein 2 (MAP2) antibody (FITC)

Antigen: Microtubule Associated Protein 2 (MAP2)

Synonyms: MAP2A, MAP2B, MAP2C, DKFZp686I2148, MAP2, MAP-2, Mtap-2, xmap2, MGC53131, a730034c02, map2, MGC145864

Clonality: Monoclonal (AP20)

Host: Mouse

Reactivity: Cow (Bovine), Chicken, Human, Mouse (Murine), Rat (Rattus), Xenopus laevis

Conjugate: FITC

Application: Immunofluorescence (IF)

Catalog no.: ABIN111293

Additional Information

Characteristics: Synonyms: Microtubule-associated protein 2, MAP2, Neuronal Marker

Alternative name: MAP-2

Gene ID: 4133

Swiss-Prot: P11137

Isotype: IgG1

Clone: AP20

Description: Microtubule Associated Protein 2 exists in two high molecular weight forms (2a and 2b)and a low molecular weight form (2c). The expression of MAP2 is developmentallyregulated and its multiple forms arise by alternative splicing of a single gene.

Specificity: Anti-MAP-2 recognizes (Mr 300 kDa)MAP-2 protein from bovine brain and dendrites andcell bodies of neurons. Anti-MAP-2 (Clone AP20) reacts with the high molecular weight forms (2a & 2b) of MAP-2but not with the low molecular weight form (2c). Species: Bovine. The antibody strongly cross-reacts with MAP-2 from human, rat, mouse,Xenopus, quail and chicken brains. Others not tested.

Application Details

Application Notes: This antibody can be used in immunohistochemistry on tissues or cell usingimmunofluorescence techniques. The suggested working dilution forimmunohistochemical staining is approximately 1: 500. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.1 mg/ml (OD280)

Buffer: 0.01 M PBS, pH 7.2, containing 2 mM EDTA, 1% BSA and 0.09% sodiumazideLabel: Fluorescein has been covalently attached to anti-MAP-2 and purified to assureoptimal fluorochrome/protein molar ratio Absorption / Emission: 492 nm / 518 nm Molar Ratio: F / P

Storage: Store the antibody at 2-8°C. DO NOT FREEZE. Protect from light. Shelf life: one year from despatch.

Research Area: Cytoskeleton

Restrictions: For Research Use only

Publications

Publications: Lewis, Ivanov, Lee et al.: "Organization of microtubules in dendrites and axons is determined by a short hydrophobic zipper in microtubule-associated proteins MAP2 and tau." in: Nature, Vol. 342, Issue 6249, pp. 498-505, 1990 (PubMed).

Peng, Binder, Black: "Biochemical and immunological analyses of cytoskeletal domains of neurons." in: The Journal of cell biology, Vol. 102, Issue 1, pp. 252-62, 1986 (PubMed).