Insulin/Proinsulin antibody

Antigen: Insulin/Proinsulin

Clonality: Monoclonal (C-PEP-01)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA)

Catalog no.: ABIN111475

Additional Information

Characteristics: Synonyms: INS

Alternative name: Insulin (+ Proinsulin)

Swiss-Prot: P01308

Immunogen: Human C-peptide conjugated to Bovine Serum Albumin

Isotype: IgG1  (Matching secondary antibodies)

Clone: C-PEP-01

Description: Proinsulin consists of the three parts: C-peptide and two long strands of amino acids(alpha and beta chains, later become linked together to form the Insulin molecule). From every molecule of Proinsulin, one molecule of Insulin plus one molecule of C-peptideare produced. C-peptide is released into the blood stream in equal amounts to Insulin.

Specificity: The antibody reacts specifically with C-peptide of Proinsulin. No cross-reactivity with Insulin or other peptide hormones or proteins was observed. Species: Human. Others not tested.

Application Details

Application Notes: ELISA. RIA. Immunocytochemistry. Immunohistochemistry on Paraffin Sections (2-5 µg/ml). Pretreatment of paraffin sectionswith 0.1% trypsin in PBS for 30 min at 37°C is recommended. Positive Control: Human pancreas (islets of Langerhans)According to customer information this antibody does not work in Western blotting. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Buffer: PBS, pH~7.4 with 15 mM Sodium Azide as preservative.

Storage: Store the antibody undiluited at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Diabetes

Restrictions: For Research Use only

Publications

Publications: Hilgert, Stolba, Kristofova et al.: "A monoclonal antibody applicable for determination of C-peptide of human proinsulin by RIA." in: Hybridoma, Vol. 10, Issue 3, pp. 379-86, 1991 (PubMed).