Goat anti-Mouse IgG3 (Heavy Chain) Antibody (Biotin) - Preadsorbed
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- Target See all IgG3 products
- IgG3
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Binding Specificity
- Heavy Chain
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Reactivity
- Mouse
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- Biotin
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Application
- ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
- Specificity
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Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG3.
Specificity was confirmed by ELISA at less than 0.5 % of target signal. - Cross-Reactivity
- Mouse (Murine)
- Characteristics
- When choosing a secondary antibody, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
- Purification
- Preadsorption: Solid phase absorption
- Immunogen
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Immunogen: Mouse IgG3 heavy chain
Immunogen Type: Native Protein
- Isotype
- IgG
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- Application Notes
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Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: Mouse IgG3 secondary antibody is available in a variety of formats. Anti-Mouse IgG3 secondary antibody is suitable for ELISA, Immunohistochemistry, western blotting as well as other anti IgG3 antibody based assays.
ELISA Dilution: 1:20,000 - 1:100,000
Western Blot Dilution: 1:2,000 - 1:10,000
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
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Avoid cycles of freezing and thawing.
Centrifuge product if not completely clear after standing at room temperature. - Storage
- RT,4 °C,-20 °C
- Storage Comment
- Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.
- Expiry Date
- 12 months
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- Target
- IgG3
- Abstract
- IgG3 Products
- Target Type
- Antibody
- Background
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Synonyms: goat anti-MOUSE IgG3 Antibody biotin Conjugation, goat anti-MOUSE IgG3 (gamma 3) biotin Conjugated Antibody
Background: Anti-Mouse IgG3 Biotin Antibody generated in goat detects reactivity to Mouse IgG3 (Gamma 3 chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG3 comprises almost 10 % of the IgG subclass and has a high affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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