Goat anti-Mouse IgG3 (Heavy Chain) Antibody (HRP) - Preadsorbed

Catalog No. ABIN1118505

Product Details

Target: IgG3

Binding Specificity: Heavy Chain

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG3.
Specificity was confirmed by ELISA at less than 0.5 % of target signal.

Characteristics: HRP Conjugated Secondary Antibodies are ideal for western blot and are available in a variety of formats and conjugate types. When choosing a secondary antibody, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Purification: Preadsorption: Solid phase absorption

Immunogen: Mouse IgG3 heavy chain
Immunogen Type: Native Protein

Isotype: IgG3

Application Details

Application Notes: Anti-Mouse IgG3 peroxidase Secondary antibody reagents are ideal for western blotting, Immunohistochemistry and ELISA as well as other antibody detection methods and anti IgG2b antibody based assays.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: Gentamicin sulfate

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.

Expiry Date: 12 months

References

Guo, Kong, Peng, Long, Zhou, Shu: "Transcription factor NF‑YA promotes a malignant phenotype by upregulating fatty acid synthase expression." in: Molecular medicine reports, Vol. 14, Issue 6, pp. 5007-5014, (2017) (PubMed).

Chen, Ruan, Long, Peng, Zhou, Liu, Zhou, Liu et al.: "Blocking fatty acid synthase inhibits tumor progression of human osteosarcoma by regulating the human epidermal growth factor receptor 2/phosphoinositide 3-kinase/protein kinase B signaling pathway ..." in: Experimental and therapeutic medicine, Vol. 13, Issue 5, pp. 2411-2416, (2017) (PubMed).

Target Details

Target: IgG3

Abstract: IgG3 Products

Target Type: Antibody