S100A9 antibody (S100 Calcium Binding Protein A9) (Biotin)

Details for Product anti-S100A9 Antibody No. ABIN111887, Supplier: Log in to see
Antigen
  • S100A9
  • 60B8AG
  • CAGB
  • CFAG
  • CGLB
  • L1AG
  • LIAG
  • MAC387
  • MIF
  • MRP14
  • NIF
  • P14
  • Mrp14
  • MRP-14
  • p14
  • 60B8Ag
  • AW546964
  • BEE22
  • Cagb
  • GAGB
  • L1Ag
  • S100 calcium binding protein A9
  • S100 calcium binding protein A9 (calgranulin B)
  • S100A9
  • S100a9
Alternatives
anti-Human S100A9 antibody for Immunohistochemistry
Reactivity
Cow (Bovine), Human
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Host
Mouse
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Clonality (Clone)
Monoclonal ()
Conjugate
This S100A9 antibody is conjugated to Biotin
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15
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Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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Options
Supplier
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Immunogen Cultured Human monocytes. Remarks: The antigen is MRP14, the epitope is suspected in the central region of the peptide.
Clone S36-48
Isotype IgG1
Specificity MRP14, granulocytes, stimulated monocytes and macrophages. Monoclonal antibody BM4026B identifies the Ca2+-binding 14 kDa subunit of the inflammatory L-1 protein complex, also called S100A9 or Calgranulin B. It is useful for the characterization of circulating granulocytes or inflammatory infiltrates of the myelo-monocytic lineage which express MRP14 differently depending on the inflammatory status of the disease. Antigen Distribution Isolated cells: The antigen is found in granulocytes and monocytes. It is absent from all other blood cells. In cultured monocytes, maximum MRP14 expression is found after 3-4 days. Myeloid leukaemic cells have been found to be positive as well. Tissue Sections: MRP14 is found in a distinct subpopulation of inflammatory perivascular infiltrates of the myelo-monocytic lineage. Macrophages synthesise MRP14 increasingly during the early stages of inflammation. A high MRP14 (and low MRP8) expression by macrophages was reported in granulomatous diseases such as tuberculosis and sarcoidis. In non-granulomatous chronic inflammatory diseases like chronic rheumatoid arthritis, MRP8 and MRP14 positive cells consist of different subpopulations. During early inflammation endothelial cells are also positive with MRP8/14 determined by antibody 27E10 (product Cat.-No BM4025).Antigen Distribution
Cross-Reactivity (Details) Species reactivity (tested):Human.The antibody reacts with Bovine spleen. It does not react with Swine tissues.
Purification Affinity Chromatography.
Alternative Name S100A9 / Calgranulin-B / MRP14 (S100A9 Antibody Abstract)
Background S100A9 is a member of the S100 family of proteins. S100A9, together with S100A8 forms a heterodimeric protein complex, Calprotectin, which is a major calcium- and zinc-binding protein in the cytosol of neutrophils, monocytes, and keratinocytes. Complexes of S100A8 and S100A9 are the physiologically relevant forms of these proteins. S100A9 may function in the inhibition of casein kinase and altered expression of this protein is associated with the disease cystic fibrosis. Its expression and potential cytokine-like function in inflammation and in cancer suggest that S100A8/A9 may play a key role in inflammation-associated cancer.Synonyms: CAGB, Calprotectin L1H subunit, Leukocyte L1 complex heavy chain, MRP-14, Migration inhibitory factor-related protein 14, S100 calcium-binding protein A9, S100-A9
Gene ID 9606
UniProt P06702
Pathways Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity
Application Notes ELISA. Immunohistochemistry on Frozen Sections: 1 μg/mL (1/200). Immunohistochemistry on Paraffin Sections: 2 μg/mL (1/100). Proteinase K pretreatmentfor antigen retrieval is recommended. Suggested Positive Control: Human tonsil. Also Suitable for Dot Blots and FACS.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Restore by adding 0.5 mL distilled water.
Concentration 0.2 mg/mL
Buffer PBS, pH 7.2 containing 10 mg/mL BSA as a stabilizer and 0.01 % Thimerosal as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Wen, Xu, Mais, Goldman, McDonnell: "The A and B isoforms of the human progesterone receptor operate through distinct signaling pathways within target cells." in: Molecular and cellular biology, Vol. 14, Issue 12, pp. 8356-64, 1994 (PubMed).

Teigelkamp, Bhardwaj, Roth, Meinardus-Hager, Karas, Sorg: "Calcium-dependent complex assembly of the myeloic differentiation proteins MRP-8 and MRP-14." in: The Journal of biological chemistry, Vol. 266, Issue 20, pp. 13462-7, 1991 (PubMed).

Delabie, de Wolf-Peeters, van den Oord, Desmet: "Differential expression of the calcium-binding proteins MRP8 and MRP14 in granulomatous conditions: an immunohistochemical study." in: Clinical and experimental immunology, Vol. 81, Issue 1, pp. 123-6, 1990 (PubMed).

Mues, Brisse, Zwadlo, Themann, Bender, Sorg: "Phenotyping of macrophages with monoclonal antibodies in endomyocardial biopsies as a new approach to diagnosis of myocarditis." in: European heart journal, Vol. 11, Issue 7, pp. 619-27, 1990 (PubMed).

Brandtzaeg, Jones, Flavell, Fagerhol: "Mac 387 antibody and detection of formalin resistant myelomonocytic L1 antigen." in: Journal of clinical pathology, Vol. 41, Issue 9, pp. 963-70, 1989 (PubMed).

Murao, Collart, Huberman: "A protein containing the cystic fibrosis antigen is an inhibitor of protein kinases." in: The Journal of biological chemistry, Vol. 264, Issue 14, pp. 8356-60, 1989 (PubMed).

Zwadlo, Brüggen, Gerhards, Schlegel, Sorg: "Two calcium-binding proteins associated with specific stages of myeloid cell differentiation are expressed by subsets of macrophages in inflammatory tissues." in: Clinical and experimental immunology, Vol. 72, Issue 3, pp. 510-5, 1988 (PubMed).

Odink, Cerletti, Brüggen, Clerc, Tarcsay, Zwadlo, Gerhards, Schlegel, Sorg: "Two calcium-binding proteins in infiltrate macrophages of rheumatoid arthritis." in: Nature, Vol. 330, Issue 6143, pp. 80-2, 1987 (PubMed).

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