Complement Membrane Cofactor Protein (CD46) antibody

Antigen: Complement Membrane Cofactor Protein (CD46)

Synonyms: MCP, TLX, AHUS2, MIC10, TRA2.10, MGC26544

Clonality: Monoclonal (13-42)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN111918

Additional Information

Characteristics: Synonyms: TLX, MIC10, Membrane cofactor protein, Trophoblast leukocyte common antigen

Alternative name: CD46 / MCP

Swiss-Prot: P15529

Immunogen: Human U-251 cells.

Isotype: IgG1  (Matching secondary antibodies)

Clone: 13-42

Description: CD46 is also known as Membrane Cofactor Protein (MCP). CD46 acts as a cofactor forfactor I, a serine protease which is involved in the degradative cleavage of C3b and C4b. Structurally as well as functionally, it is a member of the regulator of complementactivation proteins. CD46 is composed of an amino terminus of four short consensusrepeating units (SCR), a Ser/Thr (ST)-rich domain, 13 amino acid residues with unknownfunction, a transmembrane region, and a cytoplasmic tail. There are many isoforms onhuman cells and in body fluids which are distinguishable by SDS-PAGE. Theirheterogeneity stems from varying amounts of O-linked sugars secondary to alternativesplicing of mRNA coding the ST-rich region.

Specificity: The monoclonal antibody 13/42 recognizes human CD46. The recognized epitope is localized on the first two short consensus repeats. Crossreacts with African green monkey. Antigen Distribution: The antigen is widely expressed on human cells except erythrocytes .

Application Details

Protocol: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalumProtocol with formalin-fixed, paraffin-embedded sections: The whole procedure is performed at room temperature1. Deparaffinize and rehydrate tissue section2. Incubate the tissue section with proteinase K for 7min. 3. Wash in distilled water 4. Block endogenous peroxidase 5. Wash in PBS6. Block with 10% normal goat serum in PBS for 30min. in a humid chamber7. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber8. Wash in PBS9. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber10. Wash in PBS11. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 12. Wash in PBS13. Counterstain with Mayer's hemalum.

Application Notes: Suitable for Immunohistochemistry (IHC), FACS, Western Blots and Functional Studies. Recommended Dilutions: IHC on Frozen sections: 1-2 µg/ml (1/200-1/400)IHC on Paraffin sections: 8 µg/ml (1/50), Proteinase K pretreatment for antigen retrieval isrecommended. Flow Cytometry: 5-10 µg/ml of neat antibody to label 1x10e6 cells. Immunoprecipitation: 5 µg/ 200 µl extract from 2x10e6 cells. Suggested Positive Control: Human tonsil. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.4 mg/ml

Buffer: PBS, pH 7.4 with 10 mg/ml BSA as a stabilizer and 0.09% Sodium Azide asa preservative.Reconstitution: Restore with 0.5 ml distilled water.

Storage: Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer. Do not freeze working dilutionsAvoid repeated freezing and thawing. Shelf life: One year from despatch.

Research Area: CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Images

Table 1. Tissue distribution of CD46 (Johnstone et al., modified)

Publications

Publications: Johnstone, Russell, Loveland et al.: "Polymorphic expression of CD46 protein isoforms due to tissue-specific RNA splicing." in: Molecular immunology, Vol. 30, Issue 14, pp. 1231-41, 1993 (PubMed).

Schneider-Schaulies, Schnorr, Brinckmann et al.: "Receptor usage and differential downregulation of CD46 by measles virus wild-type and vaccine strains." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 9, pp. 3943-7, 1995 (PubMed).

Schneider-Schaulies, Dunster, Schwartz-Albiez et al.: "Physical association of moesin and CD46 as a receptor complex for measles virus." in: Journal of virology, Vol. 69, Issue 4, pp. 2248-56, 1995 (PubMed).

Fett, Hermann, Muether et al.: "Immunohistochemical localization of complement regulatory proteins in the human retina." in: Histology and histopathology, Vol. 27, Issue 3, pp. 357-64, 2012 (PubMed).