Insulin antibody

Catalog No. ABIN111965

The Mouse Monoclonal anti-Insulin antibody is suitable to detect Insulin in samples from Human, Pig and Cow. It has been validated for IHC (p), RIA, EIA and Func.

Quick Overview for Insulin antibody (ABIN111965)

Target: Insulin (INS)

Reactivity: Human, Pig, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This Insulin antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA), Enzyme Immunoassay (EIA), Functional Studies (Func)

Clone: IN-05

Product Details anti-Insulin Antibody

Specificity: The antibody reacts with insulin, one of the major regulatory endocrine hormones of intermediate metabolism, normally secreted by the beta cells (a type of islet cells) of the pancreas, it is also present in tumors of B cell origin such as insulinoma. The antibody IN-05 blocks binding of Insulin to the receptor.

Purification: Affinity Chromatography on Protein A.

Immunogen: Porcine Insulin

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on formalin-fixed, paraffin-embedded tissue sections. Immunocytochemistry. ELISA. RIA. Functional Applications (The antibody blocks binding of insulin to the receptor).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.3 with 15 mM Sodium Azide as presrevative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Insulin

Target: Insulin (INS)

Alternative Name: Insulin

Background: Insulin and glucagon are pancreatic endocrine hormones secreted by islet cells within the pancreas. The stimulus for insulin secretion is a HIGH blood glucose. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population.Synonyms: INS

Gene ID: 3630

NCBI Accession: NP_000198

UniProt: P01308

Pathways: NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation