CD31 antibody

Catalog No. ABIN111978

Product Details anti-CD31 Antibody

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD31 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Monoclonal CD31 antibody (clone ER-MP12) recognizes PECAM-1, platelet/endothelial cell adhesion molecule 1. It is a general marker for the detection of a major population of haematopoietic stem cells and is useful for the accumulation of early murine macrophage precursors from bone marrow cell suspensions by FACS. An even more precise characterization of such isolated and concentrated precursor cells is achieved when ER-MP12 is combined with other markers like ER-MP20 (anti Ly-6C, BM4019) or ER-MP58 (BM4089). The ER-MP12 antigen is also expressed by endothelial cells. Antigen Distribution Isolated Cells: The antigen is found on the surface of approximately 40 % of freshly isolated bone marrow cells of adult mice, on the majority of immortalized macrophage precursor cell lines (M1, RMB-1, RMB-3) corresponding to CFU-GM cells. The antibody detects pluri- and multipotent stem cells and prothymocytes as well as particular lymphoid cells in bone marrow and peripheral lymphoid organs. Tissue Sections: The antigen is detected within the lymphopoietic islands in the spleen of newborn (day 12) and in the bone marrow of adult mice. Capillary endothelial cells of adult mice also express the antigen. Detects a major population of Mouse colony forming unit macrophage (CFU-M) precursor cells, subpopulation of pre CFU-M and monoblasts, precursor cells of granulocytes.

Purification: Affinity Chromatography

Immunogen: Mouse macrophage precursor cells. Remarks: The antigen is a glutaraldehyde (0.05%) and paraformaldehye (1%) resistant 140 kD surface glycoprotein (reducing and non reducing conditions) identified as CD31. The antigen expression closely follows that of CD34.

Clone: ER-MP12

Isotype: IgG2a

Application Details

Application Notes: Immunohistochemistry on Frozen Sections: 0.25-0.5 μg/mL (1/400-1/800). Immunohistochemistry on Paraffin Sections: 20 μg/mL (1/10). Proteinase K pre-treatment for antigen retrieval is recommended. Do not use standard 4 % Formalin fixation, but max.0.05 % glutaraldehyde or 1 % paraformaldehyde! Suggested Positive Control: Mouse spleen. Has been described to work in FACS.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Protocol with Frozen, ice-cold Acetone-Fixed Sections: (The whole procedure is performed at room temperature)1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti rat IgG (H+L)minimal-cross reaction to mouse) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalumProtocol with Formalin-Fixed, Paraffin-Embedded Sections: (The whole procedure is performed at room temperature)1. Deparaffinize and rehydrate tissue section2. Incubate the tissue section with proteinase K for 7min. 3. Wash in distilled water4. Block endogenous peroxidase5. Wash in PBS6. Block with 10% normal goat serum in PBS for 30min. in a humid chamber7. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber8. Wash in PBS9. Incubate with secondary antibody (peroxidase-conjugated goat anti rat IgG (H+L)minimal-cross reaction to mouse) for 1h in a humid chamber10. Wash in PBS11. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 12. Wash in PBS13. Counterstain with Mayer's hemalum

Restrictions: For Research Use only

Handling

Reconstitution: Restore by adding 0.5 mL distilled water (= 0.2 mg/mL Stock Solution).

Concentration: 0.2 mg/mL

Buffer: Stock solution contains PBS, pH 7.2 with 0.09 % Sodium Azide as preservative and 5 mg/mL BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20 °C.

References for anti-CD31 antibody (ABIN111978)

Hellfritsch, Kirsch, Schneider, Fluege, Wortmann, Frijhoff, Dagnell, Fey, Esposito, Kölle, Pogoda, Angeli, Ingold, Kuhlencordt, Östman, Pohl, Conrad, Beck: "Knockout of mitochondrial thioredoxin reductase stabilizes prolyl hydroxylase 2 and inhibits tumor growth and tumor-derived angiogenesis." in: Antioxidants & redox signaling, Vol. 22, Issue 11, pp. 938-50, (2015) (PubMed).

Morikawa, Kajimura, Nakamura, Hishiki, Nakanishi, Yukutake, Nagahata, Ishikawa, Hattori, Takenouchi, Takahashi, Ishii, Matsubara, Kabe, Uchiyama, Nagata, Gadalla, Snyder, Suematsu: "Hypoxic regulation of the cerebral microcirculation is mediated by a carbon monoxide-sensitive hydrogen sulfide pathway." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 4, pp. 1293-8, (2012) (PubMed).

Bot, Bot, Lopez-Vales, van de Lest, Saulnier-Blache, Helms, David, van Berkel, Biessen: "Atherosclerotic lesion progression changes lysophosphatidic acid homeostasis to favor its accumulation." in: The American journal of pathology, Vol. 176, Issue 6, pp. 3073-84, (2010) (PubMed).

Bock, Onderka, Dietrich, Bachmann, Kruse, Paschke, Zahn, Cursiefen: "Bevacizumab as a potent inhibitor of inflammatory corneal angiogenesis and lymphangiogenesis." in: Investigative ophthalmology & visual science, Vol. 48, Issue 6, pp. 2545-52, (2007) (PubMed).

Li, Sainson, Shi, Leek, Harrington, Preusser, Biswas, Turley, Heikamp, Hainfellner, Harris: "Delta-like 4 Notch ligand regulates tumor angiogenesis, improves tumor vascular function, and promotes tumor growth in vivo." in: Cancer research, Vol. 67, Issue 23, pp. 11244-53, (2007) (PubMed).

Larghero, Venè, Minghelli, Travaini, Morini, Ferrari, Pfeffer, Noonan, Albini, Benelli: "Biological assays and genomic analysis reveal lipoic acid modulation of endothelial cell behavior and gene expression." in: Carcinogenesis, Vol. 28, Issue 5, pp. 1008-20, (2007) (PubMed).

Li, Otsu, Murphy, Raymond: "Developmental decrease in NMDA receptor desensitization associated with shift to synapse and interaction with postsynaptic density-95." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 23, Issue 35, pp. 11244-54, (2003) (PubMed).

Target Details for CD31

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Alternative Name: CD31 / PECAM1 (PECAM1 Products)

Synonyms: CD31 antibody, CD31/EndoCAM antibody, GPIIA' antibody, PECA1 antibody, PECAM-1 antibody, endoCAM antibody, C85791 antibody, Cd31 antibody, Pecam antibody, platelet/endothelial cell adhesion molecule 1 antibody, platelet and endothelial cell adhesion molecule 1 antibody, pecam1 antibody, PECAM1 antibody, Pecam1 antibody

Background: CD31, also known as platelet endothelial cell adhesion molecule 1 (PECAM1), is a type I integral membrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is constitutively expressed on the surface of endothelial cells, and concentrated at the junction between them. It is also weakly expressed on many peripheral lymphoid cells and platelets. CD31 has been used to measure angiogenesis in association with tumor recurrence. Other studies have also indicated that CD31 and CD34 can be used as markers for myeloid progenitor cells and recognize different subsets of myeloid leukemia infiltrates (granular sarcomas).Synonyms: End °CAM, GPIIA', PECAM-1, Platelet endothelial cell adhesion molecule

Gene ID: 10090

UniProt: Q08481

Pathways: Regulation of Actin Filament Polymerization