CD8 antibody

Catalog No. ABIN111997

Product Details anti-CD8 Antibody

Target: CD8

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD8 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody detcts T-cell subpopulation, thmocytes. Antigen distribution: Isolated cells: Subpopulation of T-cells in the peripheral blood. Tissue sections: Rib 6/1 detects the T-cell regions in lymphoid organs and stains cortical and medullary thymocytes positively. Monoclonal antibody Rib 6/1 is used for the identificaton of CD8 bearing cells, mainly Tcytotoxic and suppressor lymphocytes, in tissue sections and isolated cell suspensions. Preliminary results indicate that the Rib 6/1 antigen is the β-chain of the CD8 antigen which is not expressed by NK-cells.

Purification: Affinity chromatography

Immunogen: Rat lymphoblasts

Clone: Rib 6-1

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on frozen sections (1 μg/mL, 1: 200). Suggested positive control: rat spleen. Does not react on routinely processed paraffin sections. Flow cytometry (use 10 μL of neat antibody to label 10^6 cells).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30 min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG (H+L)minimal-cross reaction to rat) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalum

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.5 mL distilled water.

Buffer: Phosphate buffered saline pH 7.2 (PBS), 10 mg/mL bovine serum albumin (BSA) as a stabilizer and 0.01 % thimerosal as a preservative

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C/-80 °C

Storage Comment: Store (in aliquots) at -20 °C to -80 °C.

Target Details for CD8

Target: CD8

Alternative Name: CD8 (CD8 Products)

Synonyms: CD8 antibody, Leu2 antibody, MAL antibody, p32 antibody, CD8a molecule antibody, CD8A antibody

Background: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2

Gene ID: 24930

NCBI Accession: NP_113726

UniProt: P07725