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Keratin 6 antibody

This anti-Keratin 6 antibody is a Mouse Monoclonal antibody detecting Keratin 6 in IHC (p), WB and IHC (fro). Suitable for Human and Mouse. This Primary Antibody has been cited in 1 publication.
Catalog No. ABIN112170

Quick Overview for Keratin 6 antibody (ABIN112170)

Target

Keratin 6 (KRT6)

Reactivity

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Human, Mouse

Host

  • 18
  • 3
Mouse

Clonality

  • 20
  • 1
Monoclonal

Conjugate

  • 12
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This Keratin 6 antibody is un-conjugated

Application

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Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone

Ks6-KA12
  • Specificity

    The antibody is suitable for discrimination of keratinizing and non-keratinizing squamous cell carcinoma versus e. g. poorly differentiated adenocarcinoma. Clone Ks6.KA12 represents an excellent marker for non-keratinized squamous epithelia and proliferating cells of epidermis (e.g. within psoriatic lesions). Reacts with a Mr 56000 polypeptide (keratin K6, formerly also designated cytokeratin 6) of squamous epithelia. Tested Reactivities on Cultured Cell Lines: A-431, Detroit 562 and SCC-12.

    Purification

    Affinity Chromatography on Protein A.

    Immunogen

    Keratin K6 of Human callus cytoskeletal preparation.

    Isotype

    IgG1
  • Application Notes

    Immunoblotting (Western blot). Immunohistochemistry on Frozen and Paraffin Embedded Sections after formalin fixation(microwave treatment improves performance). After reconstitution, dilute the antibodyfurther 1/10 with PBS for Immunohistochemical applications. Incubation Time: 1h at RT, extended with Paraffin.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Reconstitution

    Restore in 1 mL distilled water.

    Buffer

    PBS, pH 7.4, 0.09 % Sodium Azide, 0.5 % BSA

    Preservative

    Sodium azide

    Precaution of Use

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Avoid repeated freeze-thaw cycles.

    Storage

    4 °C/-20 °C

    Storage Comment

    Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Poschmann, Sitek, Sipos, Ulrich, Wiese, Stephan, Warscheid, Klöppel, Vander Borght, Ramaekers, Meyer, Stühler: "Identification of proteomic differences between squamous cell carcinoma of the lung and bronchial epithelium." in: Molecular & cellular proteomics : MCP, Vol. 8, Issue 5, pp. 1105-16, (2009) (PubMed).

  • Target

    Keratin 6 (KRT6)

    Alternative Name

    Cytokeratin 6

    Background

    Keratins 6 and 16 are expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyperproliferation related keratins). Keratin 6 is found in hair follicles, neck squamous cell carcinomas, suprabasal cells of a variety of internal stratified epithelia, in epidermis, in both normal and hyperproliferative situations. Epidermal injury results in activation of keratinocytes which express CK6 and CK16. CK6 is strongly expressed in about 75 % of head and neck squamous cell carcinomas. Expression of CK6 is particularly associated with differentiation. There are at least six isoforms of human type II keratin 6 (K6), K6A being the most abundant representing about 77 % of all forms found in epithelia.Synonyms: Cytokeratin 6, Cytokeratin6A, Cytokeratin6D, K6, KRT-6, KRT6A, KRT6B, KRT6C, KRT6D, KRT6E, KRTL1, Keratin type II cytoskeletal 6A

    Gene ID

    3853

    NCBI Accession

    NP_005545

    UniProt

    P02538
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