KRT8 antibody

Catalog No. ABIN112171

This Mouse Monoclonal antibody specifically detects KRT8 in WB, IF, IHC (p) and IHC (fro). It exhibits reactivity toward Human, Rat, Mouse and Hamster.

Quick Overview for KRT8 antibody (ABIN112171)

Target: KRT8 (Keratin 8 (KRT8))

Reactivity: Human, Rat, Mouse, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This KRT8 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: Ks8-7

Product Details anti-KRT8 Antibody

Specificity: Ks 8.7 represents an excellent marker to discriminate simple epithelia from those of different origin. Polypetide Reacting: Mr 52500 polypeptide (keratin K8, formerly also designated cytokeratin 8) of human epithelia. Tumors Specifically Detected: All adeno-CA tested, undifferentiated CA, cervix CA, hepatocellular CA. Tested Reactivities on cultured cell lines: MCF-7, RT 112, PLC.

Purification: Protein A affinity chromatography

Immunogen: Cytoskeletal proteins from cultured HeLa cells

Isotype: IgG1

Application Details

Application Notes: Western blot. Immunocytology. Immunohistochemistry (1: 10) on frozen tissues and paraffin-embedded sections aftermicrowave treatment. Incubation time: 1 h at RT.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in 1 mL distilled water.

Buffer: Final solution contains 0.09 % Sodium Azide and 0.5 % BSA in PBS buffer, pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for KRT8

Target: KRT8 (Keratin 8 (KRT8))

Alternative Name: Cytokeratin 8

Background: Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual cytokeratin polypeptides are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.Cytokeratin 8 belongs to the type B (basic) subfamily of high molecular weight keratins and exists in combination with cytokeratin 18. Cytokeratin 8 is primarily found in the non squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin polypeptides 8 and 18.Synonyms: CK8, CYK8, Cytokeratin endo A, Cytokeratin-8, K8, KRT8, Keratin, Keratin-8, type II cytoskeletal 8

Gene ID: 3856

NCBI Accession: NP_002264

UniProt: P05787