Insulin antibody

Catalog No. ABIN112204

This Mouse Monoclonal antibody specifically detects Insulin in IHC (fro) and EIA. It exhibits reactivity toward Human, Mouse, Pig and Rat.

Quick Overview for Insulin antibody (ABIN112204)

Target: Insulin (INS)

Reactivity: Human, Mouse, Pig, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Insulin antibody is un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: 5E4-3

Product Details anti-Insulin Antibody

Specificity: The antibody is specific for insulin and proinsulin.Aff. Const.:8.1 x 10e8 M-1

Cross-Reactivity (Details): Also reacts to: Proinsulin

Purification: Affinity Chromatography on Protein A

Immunogen: Native human insulin

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on Frozen Sections. ELISA: This antibody is used as a Capture Antibody in Sandwich with BM508 (Sensitivity0.2 ng rat-mouse Insulin/mL).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Insulin

Target: Insulin (INS)

Alternative Name: Insulin

Background: Insulin is one of the major regulatory hormones of intermediate metabolism throughout the body. The biological actions of this hormone involve integration of carbohydrate, protein, and lipid metabolism. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Immunocytochemical investigations have localized insulin in the B cells of pancreatic islets of Langerhans. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of B cell origin such as insulinoma.Synonyms: INS

Gene ID: 3630

NCBI Accession: NP_000198

UniProt: P01308

Pathways: NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation